Kosior Michal Andrzej, Calabria Alfonso, Benitez Mora Maria Paz, Russo Marco, Presicce Giorgio Antonio, Cocchia Natascia, Monti Salvatore, Aardema Hilde, Gasparrini Bianca
Department of Veterinary Medicine and Animal Production, Federico II University, Via F. Delpino 1, 80137 Naples, Italy.
Faculty of Veterinary Sciences, National University of Asunción (U.N.A.), San Lorenzo 2169, Paraguay.
Animals (Basel). 2022 Aug 17;12(16):2108. doi: 10.3390/ani12162108.
The reduced oocyte competence recorded during the non-breading season (NBS) is one of the key factors affecting the profitability of buffalo farming and limits the IVEP efficiency. The purpose of this experiment was to evaluate whether season influences the lipid content within the ovarian follicle in the Italian Mediterranean buffalo. Abattoir-derived ovaries were collected during the breeding season (BS) and the NBS, and different matrices (follicular fluid, oocytes, cumulus and follicular cells) were recovered. After the extraction of the apolar fraction, all samples were analyzed by H1 nuclear magnetic resonance and FF samples by gas chromatography-mass spectrometry. Seasonal differences in lipid composition were observed in all matrices. In particular, during the NBS, the triglyceride content was higher in the follicular fluid and in the oocytes but reduced in the follicular cells. Both cholesterol and phospholipids were reduced in the follicular fluid and follicular cells during the NBS. Furthermore, the total amount of non-esterified fatty acids was significantly increased in the follicular fluid. The seasonal variation in lipid profile of the follicle may, in part, account for the reduced buffalo oocyte competence during the NBS, due to the critical role played by lipids in regulating ovarian functions.
在非繁殖季节（NBS）记录到的卵母细胞能力下降是影响水牛养殖盈利能力的关键因素之一，并限制了体外胚胎生产（IVEP）效率。本实验的目的是评估季节是否会影响意大利地中海水牛卵巢卵泡内的脂质含量。在繁殖季节（BS）和非繁殖季节收集来自屠宰场的卵巢，并回收不同的基质（卵泡液、卵母细胞、卵丘和卵泡细胞）。提取非极性部分后，所有样品通过氢-1核磁共振进行分析，卵泡液样品通过气相色谱-质谱联用仪进行分析。在所有基质中均观察到脂质组成的季节性差异。特别是在非繁殖季节，卵泡液和卵母细胞中的甘油三酯含量较高，但卵泡细胞中的甘油三酯含量降低。在非繁殖季节，卵泡液和卵泡细胞中的胆固醇和磷脂含量均降低。此外，卵泡液中非酯化脂肪酸的总量显著增加。由于脂质在调节卵巢功能中发挥的关键作用，卵泡脂质谱的季节性变化可能部分解释了非繁殖季节水牛卵母细胞能力下降的原因。
