Kosior Michal Andrzej, Esposito Riccardo, Cocchia Natascia, Piscopo Federica, Longobardi Valentina, Cacciola Nunzio Antonio, Presicce Giorgio Antonio, Campanile Giuseppe, Aardema Hilde, Gasparrini Bianca
Department of Veterinary Medicine and Animal Production - Federico II University, Via F. Delpino 1, 80137, Naples, Italy.
Department of Veterinary Medicine and Animal Production - Federico II University, Via F. Delpino 1, 80137, Naples, Italy.
Theriogenology. 2023 May;202:42-50. doi: 10.1016/j.theriogenology.2023.02.022. Epub 2023 Feb 25.
The aim of this work was to evaluate the seasonal effect on the metabolomic profile of the ovarian follicle in Italian Mediterranean buffalo to unravel the causes of the reduced competence during the non-breeding season (NBS). Samples of follicular fluid, follicular cells, cumulus cells and oocytes were collected from abattoir-derived ovaries during breeding season (BS) and NBS and analyzed by 1H Nuclear Magnetic Resonance. The Orthogonal Projections to Latent Structures of the Discriminant Analysis showed clear separation into seasonal classes and Variable Importance in Projection method identified differentially abundant metabolites between seasons. Seasonal differences were recorded in metabolite content in all analyzed components suggesting that the decreased oocyte competence during NBS may be linked to alteration of several metabolic pathways. The pathway enrichment analysis revealed that differences in the metabolites between the seasons were linked to glutathione, energy generating and amino acid metabolism and phospholipid biosynthesis. The current work allows the identification of potential positive competence markers in the follicular fluid as glutathione, glutamate, lactate and choline, and negative markers like leucine, isoleucine and β-hydroxybutyrate. These results form a major basis to develop potential strategies to optimize the follicular environment and the IVM medium to improve the competence of oocytes during the NBS.
这项工作的目的是评估季节对意大利地中海水牛卵巢卵泡代谢组学特征的影响，以揭示非繁殖季节（NBS）期间能力下降的原因。在繁殖季节（BS）和NBS期间，从屠宰场获得的卵巢中收集卵泡液、卵泡细胞、卵丘细胞和卵母细胞样本，并通过1H核磁共振进行分析。判别分析的正交投影到潜在结构显示出明显的季节分类，投影法中的变量重要性识别出了不同季节间丰度有差异的代谢物。在所有分析成分的代谢物含量中都记录到了季节差异，这表明NBS期间卵母细胞能力下降可能与多种代谢途径的改变有关。通路富集分析表明，不同季节间代谢物的差异与谷胱甘肽、能量生成、氨基酸代谢和磷脂生物合成有关。目前的工作使得能够在卵泡液中识别出潜在的正向能力标记物，如谷胱甘肽、谷氨酸、乳酸和胆碱，以及负向标记物，如亮氨酸、异亮氨酸和β-羟基丁酸。这些结果构成了制定潜在策略的主要基础，以优化卵泡环境和体外成熟培养基，从而提高NBS期间卵母细胞的能力。
