Expression of the Gene in the Soilborne Can Promote the Control Efficacy against Tobacco Bacterial Wilt.

The environmental bacterium produces antagonistic secondary metabolites with inhibitory effects on multiple plant pathogens, including the causal agent of bacterial wilt. In this study, an engineered strain was generated to express , which determines the incompatible interactions with tobacco plants. The gene, together with its native promoter, was integrated into the chromosome. The resulting strain showed no difference in antimicrobial activity against . Promoter-LacZ fusion and RT-PCR experiments demonstrated that the gene was transcribed in culture media. Compared with that of the wild type, the engineered strain reduced the disease index by 9.1% for bacterial wilt on tobacco plants. A transcriptome analysis was performed to identify differentially expressed genes in tobacco plants, and the results revealed that ethylene- and jasmonate-dependent defense signaling pathways were induced. These data demonstrates that the engineered expressing can improve biological control against tobacco bacterial wilt by the activation of host defense responses.