Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, Pernambuco, Brazil.
Centro de Tecnologias Estratégicas do Nordeste (CETENE), Recife, Pernambuco, Brazil.
Microb Ecol. 2018 Apr;75(3):555-561. doi: 10.1007/s00248-017-1073-0. Epub 2017 Sep 17.
This study describes the first antibiofilm and antibacterial screening for plants from Caatinga against Ralstonia solanacearum, a causal agent of bacterial wilt that presents serious difficulties in control. There were prepared 22 aqueous extracts of plants collected in the Vale do Catimbau-PE, Brazil. The potential antibacterial activity was evaluated by absorbance in OD and the antibiofilm activity through the crystal violet method, both of them performed in microplate against isolates of R. solanacearum biofilm formers. The results of the screening showed that Jacaranda rugosa presented antimicrobial activity higher than 90%, while Harpochilus neesianus and Myroxylon peruiferum presented antibiofilm activity higher than 50% for all tested isolates. However, Croton heliotropiifolius showed both the activities, being thus very promising for application in the control of this phytopathogen. The search for viable alternatives to the development of new bioactive compounds safe for the environment, humans, and animals from an adverse and scarce environment such as the Caatinga and encouraged us to find plants that produce effective metabolites against phytopathogenic microorganisms. This in vitro screening is important to guide the development of new products in addition to guide research studies of bioactive compounds.
本研究描述了针对茄科雷尔氏菌（一种引起细菌性萎蔫的病原体）的巴西卡廷加植物的首次抗生物膜和抗菌筛选。从巴西瓦尔·多·卡廷加（Vale do Catimbau-PE）采集了 22 种植物的水提物。通过 OD 吸光度评估潜在的抗菌活性，通过结晶紫法评估抗生物膜活性，这两种方法都在微孔板中针对形成生物膜的茄科雷尔氏菌分离株进行了测试。筛选结果表明，蓝花楹具有超过 90%的抗菌活性，而南美榛和秘鲁香脂具有超过 50%的抗生物膜活性，针对所有测试的分离株。然而，变色叶吊灯树具有这两种活性，因此在控制这种植物病原体方面具有很大的应用前景。从恶劣和稀缺的环境（如卡廷加）中寻找对环境、人类和动物安全的新生物活性化合物的可行替代品，这促使我们寻找能够产生有效代谢物对抗植物病原微生物的植物。这种体外筛选对于指导新产品的开发非常重要，此外还可以指导生物活性化合物的研究。
