Suryanto Michael Edbert, Yang Chun-Chuen, Audira Gilbert, Vasquez Ross D, Roldan Marri Jmelou M, Ger Tzong-Rong, Hsiao Chung-Der
Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 320314, Taiwan.
Department of Chemistry, Chung Yuan Christian University, Chung-Li 320314, Taiwan.
Antibiotics (Basel). 2022 Aug 4;11(8):1059. doi: 10.3390/antibiotics11081059.
Antibiotics are extensively used in aquaculture to prevent bacterial infection and the spread of diseases. Some antibiotics have a relatively longer half-life in water and may induce some adverse effects on the targeted fish species. This study analyzed the potential adverse effects of antibiotics in zebrafish at the behavioral level by a phenomic approach. We conducted three-dimensional (3D) locomotion tracking for adult zebrafish after acute exposure to twenty different antibiotics at a concentration of 100 ppb for 10 days. Their locomotor complexity was analyzed and compared by fractal dimension and permutation entropy analysis. The dimensionality reduction method was performed by combining the data gathered from behavioral endpoints alteration. Principal component and hierarchical analysis conclude that three antibiotics: amoxicillin, trimethoprim, and tylosin, displayed unique characteristics. The effects of these three antibiotics at lower concentrations (1 and 10 ppb) were observed in a follow-up study. Based on the results, these antibiotics can trigger several behavioral alterations in adult zebrafish, even in low doses. Significant changes in locomotor behavioral activity, such as total distance activity, average speed, rapid movement time, angular velocity, time in top/bottom duration, and meandering movement are highly related to neurological motor impairments, anxiety levels, and stress responses were observed. This study provides evidence based on an in vivo experiment to support the idea that the usage of some antibiotics should be carefully addressed since they can induce a significant effect of behavioral alterations in fish.
抗生素在水产养殖中被广泛用于预防细菌感染和疾病传播。一些抗生素在水中的半衰期相对较长，可能会对目标鱼类产生一些不良影响。本研究通过表型组学方法在行为水平上分析了抗生素对斑马鱼的潜在不良影响。我们对成年斑马鱼在急性暴露于浓度为100 ppb的20种不同抗生素10天后进行了三维（3D）运动跟踪。通过分形维数和排列熵分析对它们的运动复杂性进行了分析和比较。通过结合行为终点改变收集的数据进行降维方法。主成分分析和层次分析得出结论，三种抗生素：阿莫西林、甲氧苄啶和泰乐菌素表现出独特的特征。在后续研究中观察了这三种抗生素在较低浓度（1和10 ppb）下的影响。基于这些结果，这些抗生素即使在低剂量下也会引发成年斑马鱼的几种行为改变。观察到运动行为活动的显著变化，如总距离活动、平均速度、快速运动时间、角速度、在顶部/底部持续时间以及曲折运动时间，这些都与神经运动障碍、焦虑水平和应激反应高度相关。本研究基于体内实验提供了证据，以支持应谨慎对待某些抗生素的使用这一观点，因为它们会在鱼类中诱导行为改变的显著影响。
