Pani Arianna, Lucini Valeria, Dugnani Silvana, Schianchi Alice, Scaglione Francesco
Oncology and Hemato-Oncology, University of Milan, 20122 Milan, Italy.
Antibiotics (Basel). 2022 Aug 17;11(8):1114. doi: 10.3390/antibiotics11081114.
(1) Background: Cystic fibrosis (CF) is characterized by chronic pulmonary inflammation and persistent bacterial infections. is among the main opportunistic pathogens causing infections in CF. is able to form a biofilm, decreasing antibiotic permeability. LOX, a lipoxygenase enzyme, is a virulence factor produced by and promotes its persistence in lung tissues. The aim of this study is to evaluate if antibiotics currently used for aerosol therapy in CF are able to interfere with the production of lipoxygenase from open isolates of from patients with CF. (2) Methods: Clinical isolates of from patients with CF were grown in Luria broth (LB). Minimum inhibitory concentration (MIC) was performed and interpreted for all isolated strains according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. We selected four antibiotics with different mechanisms of action: aztreonam, colistin, amikacin, and levofloxacin. We used human pulmonary epithelial NCI-H929 cells to evaluate LOX activity and its metabolites according to antibiotic action at increasing concentrations. (3) Results: there is a correlation between LOX secretion by clinical isolates of and biofilm production. Levofloxacin exhibits highly significant inhibitory activity compared to the control. Amikacin also exhibits significant inhibitory activity against LOX production. Aztreonam and colistin do not show inhibitory activity. These results are also confirmed for LOX metabolites. (4) Conclusions: among the evaluated antibiotics, levofloxacin and amikacin have an activity on LOX secretion.
(1) 背景：囊性纤维化（CF）的特征是慢性肺部炎症和持续性细菌感染。[具体细菌名称]是导致CF感染的主要机会性病原体之一。它能够形成生物膜，降低抗生素通透性。脂氧合酶（LOX）是由[具体细菌名称]产生的一种毒力因子，可促进其在肺组织中的持续存在。本研究的目的是评估目前用于CF雾化治疗的抗生素是否能够干扰CF患者中[具体细菌名称]分离株脂氧合酶的产生。(2) 方法：CF患者的[具体细菌名称]临床分离株在Luria肉汤（LB）中培养。根据欧洲抗菌药物敏感性试验委员会（EUCAST）指南对所有分离菌株进行最低抑菌浓度（MIC）测定并解释结果。我们选择了四种作用机制不同的抗生素：氨曲南、黏菌素、阿米卡星和左氧氟沙星。我们使用人肺上皮NCI - H929细胞，根据抗生素在不同浓度下的作用来评估LOX活性及其代谢产物。(3) 结果：[具体细菌名称]临床分离株的LOX分泌与生物膜产生之间存在相关性。与对照组相比，左氧氟沙星表现出高度显著的抑制活性。阿米卡星对LOX产生也表现出显著的抑制活性。氨曲南和黏菌素未显示抑制活性。这些结果在LOX代谢产物方面也得到了证实。(4) 结论：在评估的抗生素中，左氧氟沙星和阿米卡星对LOX分泌有活性。
