Department of Pharmaceutical Sciences, University of Perugia, Via Romana, 06126 Perugia, Italy.
Cells. 2022 Aug 22;11(16):2617. doi: 10.3390/cells11162617.
In healthy individuals, tight junction proteins (TJPs) maintain the integrity of the intestinal barrier. Dysbiosis and increased intestinal permeability are observed in several diseases, such as inflammatory bowel disease. Many studies highlight the role of probiotics in preventing intestinal barrier dysfunction. The present study aims to investigate the effects of a commercially available probiotic formulation of LR 32, BL 04, and BB 536 (Serobioma, Bromatech s.r.l., Milan, Italy) on TJPs and the integrity of the intestinal epithelial barrier, and the ability of this formulation to prevent lipopolysaccharide-induced, inflammation-associated damage. An in vitro model of the intestinal barrier was developed using a Caco-2 cell monolayer. The mRNA expression levels of the TJ genes were analyzed using real-time PCR. Changes in the amounts of proteins were assessed with Western blotting. The effect of Serobioma on the intestinal epithelial barrier function was assessed using transepithelial electrical resistance (TEER) measurements. The probiotic formulation tested in this study modulates the expression of TJPs and prevents inflammatory damage. Our findings provide new insights into the mechanisms by which probiotics are able to prevent damage to the gut epithelial barrier.
在健康个体中，紧密连接蛋白（TJPs）维持肠道屏障的完整性。在几种疾病中，如炎症性肠病，观察到肠道菌群失调和肠道通透性增加。许多研究强调了益生菌在预防肠道屏障功能障碍中的作用。本研究旨在研究一种市售益生菌制剂 LR 32、BL 04 和 BB 536（Serobioma，Bromatech s.r.l.，米兰，意大利）对 TJPs 和肠道上皮屏障完整性的影响，以及该制剂预防脂多糖诱导的炎症相关损伤的能力。使用 Caco-2 细胞单层建立了肠道屏障的体外模型。使用实时 PCR 分析 TJ 基因的 mRNA 表达水平。使用 Western blotting 评估蛋白质含量的变化。使用跨上皮电阻（TEER）测量来评估 Serobioma 对肠道上皮屏障功能的影响。本研究中测试的益生菌制剂调节 TJPs 的表达并预防炎症损伤。我们的研究结果提供了关于益生菌能够预防肠道上皮屏障损伤的机制的新见解。
