Oncology Research Laboratory, Oncology Institute, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania.
Department of Genetics and Molecular Medicine, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania.
Genes (Basel). 2022 Jul 29;13(8):1365. doi: 10.3390/genes13081365.
Cervical cancer is one of the most common cancers in women worldwide, which is typically caused by human papillomavirus (HPV). Usually, the toll-like receptor (TLR) signaling pathways eliminate the virus from the organism, but in some cases, persistent infection may develop. Unfortunately, the mechanism of immune tolerance is still unclear. Therefore, this study aimed to analyze rs8177376, rs611953, rs3802814, and rs8177374 polymorphisms and to identify their impact on cervical cancer phenotype and prognosis. This study included 172 cervical cancer patients. Genotyping was performed using the PCR-RFLP assay. Univariate and multivariate logistic regression and Cox's regression models were applied for statistical analysis. The results revealed that older age at the time of diagnosis was statistically linked with the rs8177376 T allele (OR = 2.901, 95% Cl 1.750-4.808, = 0.000) and the rs611953 G allele (OR = 3.258, 95% Cl 1.917-5.536, = 0.000). Moreover, the T allele of rs8177376 (OR = 0.424, 95% Cl 0.220-0.816, = 0.010) was found to be statistically associated with the lower tumor grade. Thus, polymorphisms might be employed in the future as potential biomarkers for determining the phenotype and prognosis of cervical cancer.
宫颈癌是全球女性最常见的癌症之一，通常由人乳头瘤病毒（HPV）引起。通常， toll 样受体（TLR）信号通路可将病毒从体内清除，但在某些情况下，可能会发展为持续性感染。不幸的是，免疫耐受的机制仍不清楚。因此，本研究旨在分析 rs8177376、rs611953、rs3802814 和 rs8177374 多态性，并确定它们对宫颈癌表型和预后的影响。本研究纳入了 172 名宫颈癌患者。采用 PCR-RFLP 法进行基因分型。应用单因素和多因素逻辑回归和 Cox 回归模型进行统计学分析。结果表明，诊断时年龄较大与 rs8177376 的 T 等位基因（OR=2.901，95%Cl 1.750-4.808， =0.000）和 rs611953 的 G 等位基因（OR=3.258，95%Cl 1.917-5.536， =0.000）显著相关。此外，rs8177376 的 T 等位基因（OR=0.424，95%Cl 0.220-0.816， =0.010）与肿瘤分级较低显著相关。因此，多态性可能成为未来确定宫颈癌表型和预后的潜在生物标志物。
