Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation-State Research Institute, Czartoryskich 8, 24-100 Pulawy, Poland.
Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290 Lublin, Poland.
Int J Mol Sci. 2022 Aug 11;23(16):8978. doi: 10.3390/ijms23168978.
The microbial structure and metabolic function of plant-associated endophytes play a key role in the ecology of various environments, including trees. Here, the structure and functional profiles of the endophytic bacterial community, associated with × , in correlation with seasonality, were evaluated using Biolog EcoPlates. Biolog EcoPlates was used to analyse the functional diversity of the microbiome. The total communities of leaf endophyte communities were investigated using 16S rRNA V5-V7 region amplicon deep sequencing via Illumina MiSeq. Community level physiological profiling (CLPP) analysis by the Biolog EcoPlate™ assay revealed that the carboxylic acids (19.67-36.18%) and amino acids (23.95-35.66%) were preferred by all by all communities, whereas amines and amides (0.38-9.46%) were least used. Seasonal differences in substrate use were also found. Based on the sequencing data, mainly phyla Proteobacteria (18.4-97.1%) and Actinobacteria (2.29-78.7%) were identified. A core microbiome could be found in leaf-associated endophytic communities in trees growing in different locations. This work demonstrates the application of Biolog EcoPlates in studies of the functional diversity of microbial communities in a niche other than soil and shows how it can be applied to the functional analyses of endomicrobiomes. This research can contribute to the popularisation of Biolog EcoPlates for the functional analysis of the endomicrobiome. This study confirms that the analysis of the structure and function of the plant endophytic microbiome plays a key role in the health control and the development of management strategies on bioenergy tree plantations.
植物内生菌的微生物结构和代谢功能在各种环境（包括树木）的生态学中起着关键作用。在这里，通过 Illumina MiSeq 使用 16S rRNA V5-V7 区域扩增子进行深度测序，评估了与季节性相关的 × 相关内生细菌群落的结构和功能谱。使用 Biolog EcoPlates 分析微生物组的功能多样性。通过 Biolog EcoPlate™ assay 进行群落水平生理特征分析（CLPP）发现，所有群落都优先使用羧酸（19.67-36.18％）和氨基酸（23.95-35.66％），而胺和酰胺（0.38-9.46％）则使用最少。还发现了底物利用的季节性差异。根据测序数据，主要的门为 Proteobacteria（18.4-97.1％）和 Actinobacteria（2.29-78.7％）。可以在不同地点生长的树木的叶片相关内生群落中找到核心微生物组。这项工作证明了 Biolog EcoPlates 在非土壤微生物群落功能多样性研究中的应用，以及如何将其应用于内生微生物组的功能分析。这项研究有助于推广 Biolog EcoPlates 用于内生微生物组的功能分析。本研究证实，植物内生微生物组的结构和功能分析在生物能源树种种植园的健康控制和管理策略的制定中起着关键作用。
