Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, China.
Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, China; Adaptive Research Program, Quetta, 87300, Balochistan, Pakistan.
Microbiol Res. 2021 Jan;242:126639. doi: 10.1016/j.micres.2020.126639. Epub 2020 Nov 5.
Endophytic microbial-communities have specific beneficial functions and are considered key drivers for host plant health. The removing-PCR (R-PCR) is a simple culture-independent cost-effective method to identify endophytic microbial-communities. Microbial communities from maize plant grown in different soil types were identified and characterized via the R-PCR and 16S rRNA sequencing. Culture-dependent microbial community identified through 16S rRNA gene sequencing, further these bacterial communities screened for antagonistic assay against Rhizoctonia solani WH1, in vitro compatibility tests, plant-growth-promoting traits and BIOLOG identification. After that, synthetic-communities (SycomA and SycomB) were prepared by mixing different compatible bacterial-strains to use as an inoculant to suppress pathogens of maize. We identified 167 bacterial operational taxonomic units (OTUs) and unexpected 8 fungal OTUs through the R-PCR, whereas, 95 bacterial OTUs via 16S rRNA sequencing from maize leaves and roots. SycomA and SycomB treatments suppressed the disease level and promoted growth attributes more effectively as compare to the single bacterial-strain and control treatments. This study establishes an efficient approach to isolate, identify and characterize diverse endophytic microbial-community assembly in maize leaves and roots, to successfully apply particular microbes to improve crop growth in soils affected by soil-borne-pathogens.
内生微生物群落具有特定的有益功能，被认为是宿主植物健康的关键驱动因素。去除聚合酶链反应（R-PCR）是一种简单的、非培养的、具有成本效益的方法，可用于鉴定内生微生物群落。通过 R-PCR 和 16S rRNA 测序，对生长在不同土壤类型中的玉米植物的微生物群落进行了鉴定和特征描述。通过 16S rRNA 基因测序鉴定的依赖培养的微生物群落，进一步对这些细菌群落进行拮抗 Rhizoctonia solani WH1 的筛选、体外相容性测试、植物促生长特性和 BIOLOG 鉴定。之后，通过混合不同相容细菌菌株制备合成群落（SycomA 和 SycomB），用作接种剂来抑制玉米的病原菌。通过 R-PCR 我们鉴定了 167 个细菌操作分类单元（OTUs）和 8 个意想不到的真菌 OTUs，而通过 16S rRNA 测序从玉米叶片和根系中鉴定了 95 个细菌 OTUs。与单一细菌菌株和对照处理相比，SycomA 和 SycomB 处理更有效地抑制了疾病水平并促进了生长特性。本研究建立了一种有效的方法来分离、鉴定和描述玉米叶片和根系中内生微生物群落的多样性，以便成功地应用特定微生物来改善受土传病原菌影响的土壤中作物的生长。
