Institute of Food Technology, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, H-4032 Debrecen, Hungary.
Institute of Healthcare Industry, University of Debrecen, H-4032 Debrecen, Hungary.
Int J Mol Sci. 2022 Aug 12;23(16):9036. doi: 10.3390/ijms23169036.
Increased permeability of the epithelial and endothelial cell layers results in the onset of pathogenic mechanisms. In both cell types, cell-cell connections play a regulatory role in altering membrane permeability. The aim of this study was to investigate the modulating effect of anthocyanin-rich extract (AC) on TJ proteins in inflammatory Caco-2 and HUVEC monolayers. Distribution of Occludin and zonula occludens-1 (ZO-1) were investigated by immunohistochemical staining and the protein levels were measured by flow cytometry. The mRNA expression was determined by quantitative real-time PCR. The transepithelial electrical resistance (TEER) values were measured during a permeability assay on HUVEC cell culture. As a result of inflammatory induction by TNF-α, redistribution of proteins was observed in Caco-2 cell culture, which was reduced by AC treatment. In HUVEC cell culture, the decrease in protein and mRNA expression was more dominant during inflammatory induction, which was compensated for by the AC treatment. Overall, AC positively affected the expression of the examined cell-binding structures forming the membrane on both cell types.
上皮细胞和内皮细胞层通透性的增加导致了致病机制的发生。在这两种细胞类型中，细胞-细胞连接在改变膜通透性方面发挥着调节作用。本研究旨在探讨富含花色苷的提取物（AC）对炎症性 Caco-2 和 HUVEC 单层细胞中 TJ 蛋白的调节作用。通过免疫组织化学染色研究了 Occludin 和 zonula occludens-1（ZO-1）的分布，并通过流式细胞术测量了蛋白质水平。通过定量实时 PCR 确定了 mRNA 表达。在 HUVEC 细胞培养物的通透性测定期间测量了跨上皮电阻 (TEER) 值。由于 TNF-α引起的炎症诱导，在 Caco-2 细胞培养物中观察到蛋白质的重新分布，AC 处理可减少这种分布。在 HUVEC 细胞培养物中，在炎症诱导过程中，蛋白质和 mRNA 表达的减少更为明显，AC 处理可对此进行补偿。总的来说，AC 对两种细胞类型上形成膜的所检查的细胞结合结构的表达有积极影响。
