Biro Attila, Markovich Arnold, Homoki Judit Rita, Szőllősi Erzsébet, Hegedűs Csaba, Tarapcsák Szabolcs, Lukács János, Stündl László, Remenyik Judit
Institute of Animal Science, Biotechnology and Nature Conservation, Institute of Food Technology, University of Debrecen, H-4032 Debrecen, Hungary.
Department of Pharmacology and Pharmacotherapy, University of Debrecen, H-4032 Debrecen, Hungary.
Molecules. 2019 Sep 21;24(19):3427. doi: 10.3390/molecules24193427.
The anthocyanin content of Hungarian sour cherry is remarkable based on our preliminary investigations. Nutraceutical and pharmaceutical effects of anthocyanins have been extensively studied. The objective of this work was to investigate the the effect of purified sour cherry extract using human umbilical cord vein endothelial cells (HUVECs) as the inflammatory model. HUVECs were isolated by enzymatic digestion and characterized by flow cytometry. The optimal concentration range of sour cherry extract was selected based on MTT, apoptosis, and necrosis assays. Cells were divided into three groups, incubating with M199 medium as control, or with lipopolysaccharide (LPS) or with LPS plus anthocyanin extract (ACE). The effect of sour cherry extract on oxidative stress, pro-inflammatory factors, and arachidonic pathway was investigated. An amount of 50 μg/mL ACE (ACE) was able to increase the level of glutathione and decrease the ROS, thereby improving the unbalanced redox status in inflammation. ACE lowered pro-inflammatory cytokine levels including Interleukin-6 (IL-6), regulated on activation, normal T cell expressed and secreted (RANTES), granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor alpha (TNF-α). ACE affected the arachidonic acid pathway by reducing the LPS-induced enzyme expression (cyclooxygenase-1, cyclooxygenase-2, and prostacyclin synthase). The extract under investigation seems to have a pleiotropic effect including anti-oxidative, anti-inflammatory, hemostatic, and vasoactive effects. Our results indicate that purified sour cherry extract could reduce the LPS-induced inflammatory response, thereby improving endothelial dysfunction.
根据我们的初步调查，匈牙利酸樱桃的花青素含量显著。花青素的营养保健和药用作用已得到广泛研究。这项工作的目的是使用人脐静脉内皮细胞（HUVECs）作为炎症模型，研究纯化的酸樱桃提取物的效果。通过酶消化分离HUVECs，并通过流式细胞术进行表征。基于MTT、凋亡和坏死试验选择酸樱桃提取物的最佳浓度范围。将细胞分为三组，分别用M199培养基作为对照，或用脂多糖（LPS），或用LPS加花青素提取物（ACE）进行孵育。研究了酸樱桃提取物对氧化应激、促炎因子和花生四烯酸途径的影响。浓度为50μg/mL的ACE能够提高谷胱甘肽水平并降低活性氧，从而改善炎症中失衡的氧化还原状态。ACE降低了促炎细胞因子水平，包括白细胞介素-6（IL-6）、活化调节正常T细胞表达和分泌因子（RANTES）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和肿瘤坏死因子α（TNF-α）。ACE通过降低LPS诱导的酶表达（环氧化酶-1、环氧化酶-2和前列环素合酶）影响花生四烯酸途径。所研究的提取物似乎具有多效性作用，包括抗氧化、抗炎、止血和血管活性作用。我们的结果表明，纯化的酸樱桃提取物可以降低LPS诱导的炎症反应，从而改善内皮功能障碍。
