Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia.
Department of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia.
Int J Mol Sci. 2022 Aug 13;23(16):9072. doi: 10.3390/ijms23169072.
Diabetes, a chronic group of medical disorders characterized byhyperglycemia, has become a global pandemic. Some hormones may influence the course and outcome of diabetes, especially if they potentiate the formation of reactive oxygen species (ROS). There is a close relationship between thyroid disorders and diabetes. The main objective of this investigation was to find out whether peripheral blood mononuclear cells (PBMCs) are more prone to DNA damage by triiodothyronine (T) (0.1, 1 and 10 μM) at various stages of progression through diabetes (obese, prediabetics, and type 2 diabetes mellitus-T2DM persons). In addition, some biochemical parameters of oxidative stress (catalase-CAT, thiobarbituric acid reactive substances-TBARS) and lactate dehydrogenase (LDH) were evaluated. PBMCs from prediabetic and diabetic patients exhibited increased sensitivity for T regarding elevated level of DNA damage, inhibition of catalase, and increase of TBARS and LDH. PBMCs from obese patients reacted in the same manner, except for DNA damage. The results of this study should contribute to a better understanding of the role of thyroid hormones in the progression of T2DM.
糖尿病是一组以高血糖为特征的慢性医学疾病，已成为全球性的大流行病。一些激素可能会影响糖尿病的病程和结果，特别是如果它们增强活性氧（ROS）的形成。甲状腺疾病与糖尿病之间存在密切关系。本研究的主要目的是了解外周血单核细胞（PBMC）在糖尿病（肥胖、糖尿病前期和 2 型糖尿病-T2DM 患者）进展的各个阶段（肥胖、糖尿病前期和 2 型糖尿病-T2DM 患者）是否更容易受到三碘甲状腺原氨酸（T）（0.1、1 和 10 μM）的 DNA 损伤。此外，还评估了一些氧化应激的生化参数（过氧化氢酶-CAT、硫代巴比妥酸反应物质-TBARS）和乳酸脱氢酶（LDH）。糖尿病前期和糖尿病患者的 PBMC 对 T 升高水平的 DNA 损伤、过氧化氢酶抑制以及 TBARS 和 LDH 的增加更为敏感。肥胖患者的 PBMC 也有同样的反应，除了 DNA 损伤。本研究的结果应该有助于更好地了解甲状腺激素在 T2DM 进展中的作用。
