State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, China.
College of Forestry, Guizhou University, Guiyang 550025, China.
Int J Mol Sci. 2022 Aug 14;23(16):9106. doi: 10.3390/ijms23169106.
CK1 casein kinases are well conserved in filamentous fungi. However, their functions are not well characterized in plant pathogens. In , deletion of caused severe growth defects and loss of conidiation, fertility, and pathogenicity. Interestingly, the mutant was not stable and often produced fast-growing spontaneous suppressors. Suppressor mutations were frequently identified in the gene by sequencing analyses. Deletion of the entire or disruptions of its conserved C-terminal region could suppress the defects of in hyphal growth and conidiation, indicating the genetic relationship between and . Furthermore, the mutant showed defects in polarized growth, cell wall integrity, internalization of FgRho1 and vacuole fusion, which were all partially suppressed by deletion of . Overall, our results indicate a stage-specific functional relationship between and , possibly via FgRho1 signaling for regulating polarized hyphal growth and cell wall integrity.
CK1 酪蛋白激酶在丝状真菌中高度保守。然而，它们在植物病原体中的功能尚未得到很好的表征。在 中，缺失 导致严重的生长缺陷和丧失产孢、育性和致病性。有趣的是，突变体不稳定，经常产生快速生长的自发抑制子。通过测序分析，在 基因中经常鉴定到抑制子突变。缺失整个 或破坏其保守的 C 端区域可以抑制 对菌丝生长和产孢的缺陷，表明 与 之间存在遗传关系。此外，突变体在极化生长、细胞壁完整性、FgRho1 内化和液泡融合方面表现出缺陷，这些缺陷部分被 的缺失所抑制。总的来说，我们的结果表明 与 之间存在特定阶段的功能关系，可能通过 FgRho1 信号通路调节极化菌丝生长和细胞壁完整性。
