UPM 暴露的上皮细胞与巨噬细胞和树突状细胞共培养在阻塞性肺疾病中的 RNA-Seq 分析。
RNA-Seq Analysis of UPM-Exposed Epithelium Co-Cultivated with Macrophages and Dendritic Cells in Obstructive Lung Diseases.
机构信息
Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, 02-091 Warsaw, Poland.
Postgraduate School of Molecular Medicine, Medical University of Warsaw, 02-091 Warsaw, Poland.
出版信息
Int J Mol Sci. 2022 Aug 15;23(16):9125. doi: 10.3390/ijms23169125.
BACKGROUND
Elevated concentrations of airborne pollutants are correlated with an enlarged rate of obstructive lung disease morbidity as well as acute disease exacerbations. This study aimed to analyze the epithelium mRNA profile in response to airborne particulate matter in the control, asthma, and COPD groups.
RESULTS
A triple co-culture of nasal epithelium, monocyte-derived macrophages, and monocyte-derived dendritic cells obtained from the controls, asthma, and COPD were exposed to urban particulate matter (UPM) for 24 h. RNA-Seq analysis found differences in seven (CYP1B1, CYP1B1-AS1, NCF1, ME1, LINC02029, BPIFA2, EEF1A2), five (CYP1B1, ARC, ENPEP, RASD1, CYP1B1-AS1), and six (CYP1B1, CYP1B1-AS1, IRF4, ATP1B2, TIPARP, CCL22) differentially expressed genes between UPM exposed and unexposed triple co-cultured epithelium in the control, asthma, and COPD groups, respectively. PCR analysis showed that mRNA expression of BPIFA2 and ENPEP was upregulated in both asthma and COPD, while the expression of CYP1B1-AS1 and TIPARP was increased in the epithelium from COPD patients only. Biological processes changed in UPM exposed triple co-cultured epithelium were associated with epidermis development and epidermal cell differentiation in asthma and with response to toxic substances in COPD.
CONCLUSIONS
The biochemical processes associated with pathophysiology of asthma and COPD impairs the airway epithelial response to UPM.
背景
空气中污染物浓度的升高与阻塞性肺部疾病发病率的增加以及急性疾病恶化有关。本研究旨在分析对照、哮喘和 COPD 组中空气中颗粒物作用下的上皮细胞 mRNA 谱。
结果
从对照、哮喘和 COPD 患者中获得的鼻上皮、单核细胞衍生的巨噬细胞和单核细胞衍生的树突状细胞的三重共培养物暴露于城市颗粒物 (UPM) 24 小时。RNA-Seq 分析发现，在对照、哮喘和 COPD 组中，暴露于 UPM 和未暴露于 UPM 的三重共培养上皮细胞之间存在 7 个（CYP1B1、CYP1B1-AS1、NCF1、ME1、LINC02029、BPIFA2、EEF1A2）、5 个（CYP1B1、ARC、ENPEP、RASD1、CYP1B1-AS1）和 6 个（CYP1B1、CYP1B1-AS1、IRF4、ATP1B2、TIPARP、CCL22）差异表达基因。PCR 分析显示，BPIFA2 和 ENPEP 的 mRNA 表达在哮喘和 COPD 中均上调，而 CYP1B1-AS1 和 TIPARP 的表达仅在 COPD 患者的上皮细胞中增加。暴露于 UPM 的三重共培养上皮细胞中改变的生物学过程与哮喘中的表皮发育和表皮细胞分化以及 COPD 中的对有毒物质的反应有关。
结论
与哮喘和 COPD 病理生理学相关的生化过程会损害气道上皮对 UPM 的反应。
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