Lukiw W J, Kruck T P, McLachlan D R
Neurotoxicology. 1987 Summer;8(2):291-301.
Sodium cations were employed to electrostatically displace chromosomal proteins from DNA. Increasing the ambient ionic concentration resulted in a characteristic dissociation pattern of chromatin which was analyzed on polyacrylamide gels. This method of sodium chloride extraction of nuclei in vitro has been used to investigate the state of compaction of chromatin in normal and Alzheimer affected neocortical nuclei and in control nuclei treated with aluminum lactate in vitro. Our results indicate that by employing specific chelating agents such as desferrioxamine in vitro, nuclear bound aluminum is particularly resistant to removal by chelation and an increase in the affinity of linker histones H1 and H1 degree for DNA occurs both in Alzheimer's disease and in the presence of aluminum salts in vitro.
使用钠离子从DNA上静电置换染色体蛋白。提高环境离子浓度会导致染色质呈现出一种特征性的解离模式，该模式在聚丙烯酰胺凝胶上进行分析。这种体外氯化钠提取细胞核的方法已被用于研究正常和患阿尔茨海默病的新皮质细胞核以及体外经乳酸铝处理的对照细胞核中染色质的压缩状态。我们的结果表明，在体外使用去铁胺等特定螯合剂时，核结合铝特别难以通过螯合作用去除，并且在阿尔茨海默病以及体外存在铝盐的情况下，连接组蛋白H1和H1°与DNA的亲和力都会增加。
