Univ. Lille, Inserm, CHU Lille, U1286-INFINITE-Institute for Translational Research in Inflammation, F-59000 Lille, France.
Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-Center for Infection and Immunity of Lille, F-59000 Lille, France.
Int J Mol Sci. 2022 Aug 16;23(16):9207. doi: 10.3390/ijms23169207.
Microbial agents have promise for the bioremediation of Pb(II)-polluted environments and wastewater, the biodecontamination of foods, and the alleviation of toxicity in living organisms. The dairy bacterium Propionibacterium freudenreichii is poorly able to remove Pb(II) from aqueous solution at 25 ppm, ranging from 0 to 10% of initial concentration. Here, we report on an original strong enhancement of this activity (ranging from 75% to 93%, p < 0.01) following the addition of a polysorbate detergent (Tween® 80) during or either shortly after the growth of a P. freudenreichii culture. We evaluated the optimal Tween® 80 concentration for pretreatment conditions, documented the role of other detergents, and explored the possible mechanisms involved. Our results reveal a novel, environmentally friendly, low-cost pretreatment procedure for enhancing the selective removal of lead from water by probiotic-documented bacteria.
微生物制剂有望用于修复 Pb(II) 污染的环境和废水、食品的生物净化以及减轻生物体内的毒性。乳制品细菌丙酸杆菌从 25ppm 的水溶液中去除 Pb(II) 的能力很差，初始浓度范围为 0 到 10%。在这里，我们报告了在丙酸杆菌培养过程中或培养结束后添加一种聚山梨酯清洁剂（吐温® 80）后，该活性得到了显著增强（75%到 93%，p<0.01）。我们评估了最佳的吐温® 80 浓度以用于预处理条件，记录了其他清洁剂的作用，并探讨了可能涉及的机制。我们的结果揭示了一种新颖的、环保的、低成本的预处理方法，可增强益生菌细菌对水中铅的选择性去除。
