Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, University Hospital Frankfurt, Goethe University, 60590 Frankfurt am Main, Germany.
Department of Anesthesiology, Heidelberg University Hospital, 69120 Heidelberg, Germany.
Int J Mol Sci. 2022 Aug 18;23(16):9323. doi: 10.3390/ijms23169323.
The biomedical consequences of allogeneic blood transfusions and the possible pathomechanisms of transfusion-related morbidity and mortality are still not entirely understood. In retrospective studies, allogeneic transfusion was associated with increased rates of cancer recurrence, metastasis and death in patients with colorectal cancer. However, correlation does not imply causation. The purpose of this study was to elucidate this empirical observation further in order to address insecurity among patients and clinicians. We focused on the in vitro effect of microparticles derived from red blood cell units (RMPs). We incubated different colon carcinoma cells with RMPs and analyzed their effects on growth, invasion, migration and tumor marker expression. Furthermore, effects on Wnt, Akt and ERK signaling were explored. Our results show RMPs do not seem to affect functional and phenotypic characteristics of different colon carcinoma cells and did not induce or inhibit Wnt, Akt or ERK signaling, albeit in cell culture models lacking tumor microenvironment. Allogeneic blood transfusions are associated with poor prognosis, but RMPs do not seem to convey tumor-enhancing effects. Most likely, the circumstances that necessitate the transfusion, such as preoperative anemia, tumor stage, perioperative blood loss and extension of surgery, take center stage.
异体输血的生物医学后果以及与输血相关发病率和死亡率的可能发病机制仍不完全清楚。在回顾性研究中，异体输血与结直肠癌患者癌症复发、转移和死亡的发生率增加相关。然而，相关性并不意味着因果关系。本研究的目的是进一步阐明这一经验观察结果，以解决患者和临床医生的担忧。我们专注于源自红细胞单位的微粒（RMPs）的体外效应。我们将不同的结肠癌细胞与 RMP 一起孵育，并分析它们对生长、侵袭、迁移和肿瘤标志物表达的影响。此外，还探讨了对 Wnt、Akt 和 ERK 信号的影响。我们的结果表明，RMP 似乎不会影响不同结肠癌细胞的功能和表型特征，也不会诱导或抑制 Wnt、Akt 或 ERK 信号，尽管在缺乏肿瘤微环境的细胞培养模型中是如此。异体输血与预后不良相关，但 RMP 似乎不会传递促进肿瘤的作用。很可能是需要输血的情况，如术前贫血、肿瘤分期、围手术期失血和手术范围，占据了中心位置。
