Li Sizheng, Li Xiya, Li He
Key Laboratory of National Forestry, Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, China.
Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha 410004, China.
J Fungi (Basel). 2022 Aug 10;8(8):835. doi: 10.3390/jof8080835.
is an edible oil tree species native to China. Anthracnose is a common disease of , which reduces the production of the trees and brings huge economic losses. We have previously identified the fungus as the major pathogen of anthracnose in . The retromer complex participates in the intracellular retrograde transport of the cargos from the endosome to the trans-Golgi network in the eukaryotes. Vacuolar protein sorting 29 is a subunit of the retromer complex. Targeted gene deletion revealed that CfVps29 is involved in growth, conidiation, and the response to cell wall stress. We further found that the Δ mutant was minimally pathogenic to leaves, as a result of its defect in appressorium formation. This study illustrated the crucial functions of CfVps29 in the development, cell wall stress response, and pathogenicity of and, therefore, identified it as a potential fungicide target for the control of anthracnose.
是一种原产于中国的食用油树种。炭疽病是该树种的常见病害，会降低树木产量并带来巨大经济损失。我们之前已鉴定出该真菌是炭疽病的主要病原菌。回收蛋白复合体参与真核生物中货物从内体到反式高尔基体网络的细胞内逆行运输。液泡蛋白分选29是回收蛋白复合体的一个亚基。靶向基因缺失表明CfVps29参与生长、分生孢子形成以及对细胞壁应激的反应。我们进一步发现，Δ突变体对叶片致病性极低，这是由于其附着胞形成存在缺陷。本研究阐明了CfVps29在该菌的发育、细胞壁应激反应和致病性中的关键作用，因此将其确定为控制炭疽病的潜在杀菌剂靶点。
