Gan Rongcun, Zhang Shengpei, Li He
Key Laboratory of National Forestry, Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, China.
Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha 410004, China.
J Fungi (Basel). 2023 Jun 1;9(6):643. doi: 10.3390/jof9060643.
, a woody plant that produces edible oil, is indigenous to China. The devastating disease of anthracnose inflicts significant financial losses on . The primary causative agent of anthracnose on is . Chitin, a pivotal constituent of fungal cell walls, assumes a critical function in their proliferation and maturation. To study the biological functions of chitin synthase 1(Chs1) in , the gene knockout mutants, ∆-1 and ∆-2, and their complementary strain, ∆, of were generated. Our results showed that the colony diameters of wild-type and complement-strain ∆, mutant ∆-1 and ∆-2 cultured on the CM and MM medium were 5.2, 5.0, 2.2 and 2.4 cm and 4.0, 4.0, 2.1 and 2.6 cm, respectively, which were significantly smaller for the mutant than for the wild type and complement strain; the inhibition rates on the CM medium supplemented with HO, DTT, SDS and CR were 87.0% and 88.5%, 29.6% and 27.1%, 88.0% and 89.4%, and 41.7% and 28.7%, respectively, for the mutant strains, ∆-1 and ∆-2, which were significantly higher than those for the other two strains; the rate of hyphal tips with CFW fluorescence in ∆-1 and ∆-2 was 13.3% and 15.0%, which was significantly lower than those for the other two strains; the mutant strains, ∆-1 and ∆-2, lost the ability to produce conidia; the mutant strains showed weaker pathogenicity on wounded and unwounded leaves than the wild type and complement strain. The findings of this study suggest that CfChs1 plays a crucial role in the growth and development, stress responses, and pathogenicity of . Thus, this gene could be a potential target for developing novel fungicide.
一种产食用油的木本植物原产于中国。炭疽病这种毁灭性病害给[植物名称]造成了重大经济损失。[植物名称]上炭疽病的主要致病因子是[致病因子名称]。几丁质是真菌细胞壁的关键成分，在其增殖和成熟过程中发挥着关键作用。为了研究几丁质合酶1（Chs1）在[植物名称]中的生物学功能，构建了[植物名称]的基因敲除突变体∆-1和∆-2及其互补菌株∆。我们的结果表明，在CM和MM培养基上培养的野生型和互补菌株∆、突变体∆-1和∆-2的菌落直径分别为5.2、5.0、2.2和2.4厘米以及4.0、4.0、2.1和2.6厘米，突变体的菌落直径显著小于野生型和互补菌株；在添加了HO、DTT、SDS和CR的CM培养基上，突变菌株∆-1和∆-2的抑制率分别为87.0%和88.5%、29.6%和27.1%、88.0%和89.4%以及41.7%和28.7%，显著高于其他两个菌株；∆-1和∆-2中具有CFW荧光的菌丝尖端比例分别为13.3%和15.0%，显著低于其他两个菌株；突变菌株∆-1和∆-2失去了产生分生孢子的能力；突变菌株在有伤口和无伤口的[植物名称]叶片上的致病性比野生型和互补菌株弱。本研究结果表明，CfChs1在[植物名称]的生长发育、应激反应和致病性中起关键作用。因此，该基因可能是开发新型杀菌剂的潜在靶点。
