Kneißle Katharina, Krämer Markus, Kissmann Ann-Kathrin, Xing Hu, Müller Franziska, Amann Valerie, Noschka Reiner, Gottschalk Kay-Eberhard, Bozdogan Anil, Andersson Jakob, Weil Tanja, Spellerberg Barbara, Stenger Steffen, Rosenau Frank
Institute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
Max Planck Institute for Polymer Research Mainz, Ackermannweg 10, 55128 Mainz, Germany.
J Fungi (Basel). 2022 Aug 15;8(8):856. doi: 10.3390/jof8080856.
Easy and reliable identification of pathogenic species such as yeasts, emerging as problematic microbes originating from the genus , is a task in the management and treatment of infections, especially in hospitals and other healthcare environments. Aptamers are seizing an already indispensable role in different sensing applications as binding entities with almost arbitrarily tunable specificities and optimizable affinities. Here, we describe a polyclonal SELEX library that not only can specifically recognize and fluorescently label cells, but is also capable to differentiate , and cells in flow-cytometry, fluorometric microtiter plate assays and fluorescence microscopy from human cells, exemplified here by human dermal fibroblasts. This offers the opportunity to develop diagnostic tools based on this library. Moreover, these specific and robust affinity molecules could also serve in the future as potent binding entities on biomaterials and as constituents of technical devices and will thus open avenues for the development of cost-effective and easily accessible next generations of electronic biosensors in clinical diagnostics and novel materials for the specific removal of pathogenic cells from human bio-samples.
准确且可靠地鉴定致病物种，如酵母（作为源自该属的有问题微生物而出现），是感染管理和治疗中的一项任务，尤其是在医院和其他医疗环境中。适体作为具有几乎可任意调节特异性和可优化亲和力的结合实体，在不同的传感应用中正发挥着不可或缺的作用。在此，我们描述了一种多克隆SELEX文库，它不仅能够特异性识别并荧光标记细胞，而且在流式细胞术、荧光微量滴定板测定法和荧光显微镜检查中，能够将细胞与人类细胞（在此以人皮肤成纤维细胞为例）区分开来，其中包括细胞、细胞和细胞。这为基于该文库开发诊断工具提供了机会。此外，这些特异性强且稳定的亲和分子未来还可作为生物材料上的有效结合实体以及技术设备的组成部分，从而为开发经济高效且易于获取的下一代临床诊断电子生物传感器以及用于从人类生物样本中特异性去除致病细胞的新型材料开辟道路。
