Villanueva Moisés, Espinosa-Reyes Guillermo, Flores-Ramirez Rogelio, Rojas-Velazquez Angel Natanael, López Juan Carlos García, Vazquez-Valladolid Anayeli, Roque-Jimenez José Alejandro, Mendoza-Martinez German D, Hernandez-Garcia Pedro A, Palacios-Martinez Monika, Chay-Canul Alfonso J, Lee-Rangel Héctor A
Facultad de Agronomía y Veterinaria, Centro de Biociencias, Instituto de Investigaciones en Zonas Desérticas, Universidad Autónoma de San Luis Potosí, S.L.P., Soledad de Graciano Sánchez 78000, Mexico.
Facultad de Medicina-CIACYT, Centro de Investigación Aplicada en Ambiente y Salud, Universidad Autónoma de San Luis Potosí, Lomas Segunda Sección, San Luis Potosí 78210, Mexico.
Life (Basel). 2022 Aug 16;12(8):1243. doi: 10.3390/life12081243.
This experiment aimed to evaluate the effects of herbal vitamin C at different levels on tilapia ( spp.) growth, potential DNA damage, and the metabolomic profile of water effluent. Forty-five tilapias were housed in separate plastic tanks (80 L), and these were randomly assigned to three treatments: (a) a commercial diet (CD) only; (Nutripec Purina); (b) the commercial diet plus 250 mg of herbal vitamin C (HVC)/kg (CD250); and (c) the commercial diet plus 500 mg of HVC/kg (CD500). Biometric measurements were taken each week, blood samples were collected from the caudal vein on the final day, and water effluent was taken each week and immediately frozen (-80 °C) until further analysis (gas chromatography/mass spectrometry (GC/MS) systems). Data were completely randomized with a 2 × 2 factorial arrangement of treatments. Upon including herbal vitamin C, the final BW ( = 0.05) and BWG ( = 0.06) increased linearly. Herbal vitamin C decreases DNA damage ( ≥ 0.05). PLS-DA showed a 41.6% variation between treatments in the water samples. Fifteen metabolites had the best association between treatments, with a stronger correlation with CD500. Herbal vitamin C could improve fish performance, prevent DNA damage, and influence changes in the metabolomic profile of the water.
本实验旨在评估不同水平的草本维生素C对罗非鱼生长、潜在DNA损伤及排水代谢组学特征的影响。45条罗非鱼饲养在单独的塑料水箱（80升）中，随机分为三组处理：（a）仅投喂商业饲料（CD）（Nutripec Purina）；（b）商业饲料加250毫克/千克草本维生素C（HVC）（CD250）；（c）商业饲料加500毫克/千克HVC（CD500）。每周进行生物测量，在最后一天从尾静脉采集血样，每周采集排水并立即冷冻（-80℃）直至进一步分析（气相色谱/质谱（GC/MS）系统）。数据采用2×2析因处理完全随机化。添加草本维生素C后，末重（P = 0.05）和增重（P = 0.06）呈线性增加。草本维生素C可降低DNA损伤（P≥0.05）。偏最小二乘判别分析（PLS-DA）显示水样处理间差异为41.6%。15种代谢物在处理间相关性最佳，与CD500相关性更强。草本维生素C可改善鱼类生长性能，预防DNA损伤，并影响排水代谢组学特征变化。
