Duan Xiangke, Huang Xue, Xu Junqi, Li Xue, Niu Jingjing, Du Xiaoli, Wang Xiaoyu, Li Jiang, Kelly Michael, Guo Jiaohan, Zhang Ke, Huang Yu, Kan Biao, Xie Jianping
School of Life Sciences, Southwest University, Chongqing 400715, China.
Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
Microorganisms. 2022 Jul 29;10(8):1535. doi: 10.3390/microorganisms10081535.
Programmed cell death (PCD) is the result of an intracellular program and is accomplished by a regulated process in both prokaryotic and eukaryotic organisms. Here, we report a programed cell death process in , an Actinobacteria species which involves a transcription factor and a DNase of the HNH family. We found that over-expression of an ArsR family member of the transcription factor, leads to cell death. Transcriptome analysis revealed an increase in the genes' transcripts involved in DNA repair and homologous recombination, and in three members of HNH family DNases. Knockout of one of the DNase genes, , alleviated cell death and its over-expression of programmed cell death. Purified MSMEG_1275 cleaved the DNA at multiple sites. Overall, our results indicate that the MSMEG_6762 affects cell death and is mediated, at least partially, by activation of the HNH nuclease expression under a stress condition.
程序性细胞死亡（PCD）是细胞内程序的结果，并且在原核生物和真核生物中均通过一个受调控的过程来完成。在此，我们报道了在一种放线菌属物种中发生的程序性细胞死亡过程，该过程涉及一个转录因子和一个HNH家族的脱氧核糖核酸酶。我们发现转录因子ArsR家族成员的过表达会导致细胞死亡。转录组分析显示参与DNA修复和同源重组的基因转录本以及HNH家族脱氧核糖核酸酶的三个成员有所增加。敲除其中一个脱氧核糖核酸酶基因会减轻细胞死亡及其程序性细胞死亡的过表达。纯化的MSMEG_1275在多个位点切割了DNA。总体而言，我们的结果表明MSMEG_6762影响细胞死亡，并且至少部分是通过在应激条件下激活HNH核酸酶表达来介导的。
