Thi Mo Luong, Irina Puntus, Natalia Suzina, Irina Nechaeva, Lenar Akhmetov, Andrey Filonov, Ekaterina Akatova, Sergey Alferov, Olga Ponamoreva
Department of Biotechnology, Tula State University, Prospekt Lenina 92, 300012 Tula, Russia.
Russian-Vietnamese Tropical Research and Technology Center (Southern Branch), No. 1-3, 3 Thang 2 (the 3rd of February) Street, 11th Ward, District 10, Ho Chi Minh City 740500, Vietnam.
Microorganisms. 2022 Aug 8;10(8):1594. doi: 10.3390/microorganisms10081594.
The aim of our study was to reveal the peculiarities of the adaptation of rhodococci to hydrophobic hydrocarbon degradation at low temperatures when the substrate was in solid states. The ability of actinobacteria (strains X5 and S67) to degrade hexadecane at 10 °C (solid hydrophobic substrate) and 26 °C (liquid hydrophobic substrate) is described. Despite the solid state of the hydrophobic substrate at 10 °C, bacteria demonstrate a high level of its degradation (30-40%) within 18 days. For the first time, we show that specialized cellular structures are formed during the degradation of solid hexadecane by at low temperatures: intracellular multimembrane structures and surface vesicles connected to the cell by fibers. The formation of specialized cellular structures when bacteria are grown on solid hexadecane is an important adaptive trait, thereby contributing to the enlargement of a contact area between membrane-bound enzymes and a hydrophobic substrate.
我们研究的目的是揭示在底物处于固态时，红球菌在低温下适应疏水性碳氢化合物降解的特性。描述了放线菌（菌株X5和S67）在10°C（固体疏水底物）和26°C（液体疏水底物）下降解十六烷的能力。尽管在10°C时疏水底物为固态，但细菌在18天内仍表现出高水平的降解（30 - 40%）。我们首次表明，在低温下细菌降解固体十六烷的过程中会形成特殊的细胞结构：细胞内多膜结构和通过纤维与细胞相连的表面囊泡。细菌在固体十六烷上生长时形成特殊的细胞结构是一种重要的适应性特征，从而有助于扩大膜结合酶与疏水底物之间的接触面积。
