Solyanikova I P, Suzina N E, Emelyanova E V, Polivtseva V N, Pshenichnikova A B, Lobanok A G, Golovleva L A
Mikrobiologiia. 2017 Mar-Apr;86(2):188-200.
Ability of actinobacteria Rhodococcus opacus 1CP to survive under unfavorable conditions and retain its biodegradation activity was assessed. The morphological and ultrastructural features of R. opacus 1CP cells degrading benzoate in the presence of oxidants and stress-protecting agents were investigated. The cells of R. opacus 1CP were resistant to oxidative stress caused by up to 100 mM H2O2 or up to 25 μM juglone (5-oxy-1,4-naphthoquinone). After 2 h of stress impact, changes in the fatty acid composition, increased activity of antioxidant enzymes, and changes in cell morphology and ultrastructure were observed. The strain retained its ability to degrade benzoate. Quercetin had a protective effect on benzoate-degrading cells of R. opacus 1CP. The strategy for cells survival under unfavorable conditions was formulated, which included decreased cell size/volume and formation of densely-packed cell conglomerates, in which the cells are embedded into a common matrix. Formation of conglomerates may probably be considered as a means for protecting the cells against aggressive environmental factors. The multicellular conglomerate structure and the matrix material impede the penetration of toxic substances into the conglomerates, promoting survival of the cells located inside.
评估了放线菌红平红球菌1CP在不利条件下存活并保持其生物降解活性的能力。研究了在存在氧化剂和应激保护剂的情况下，红平红球菌1CP细胞降解苯甲酸盐的形态和超微结构特征。红平红球菌1CP细胞对高达100 mM过氧化氢或高达25 μM胡桃醌（5-氧基-1,4-萘醌）引起的氧化应激具有抗性。在应激影响2小时后，观察到脂肪酸组成的变化、抗氧化酶活性的增加以及细胞形态和超微结构的变化。该菌株保留了其降解苯甲酸盐的能力。槲皮素对红平红球菌1CP的苯甲酸盐降解细胞具有保护作用。制定了细胞在不利条件下的存活策略，包括细胞大小/体积减小和形成紧密堆积的细胞聚集体，其中细胞嵌入共同的基质中。聚集体的形成可能被视为保护细胞免受恶劣环境因素影响的一种方式。多细胞聚集体结构和基质材料阻碍有毒物质渗透到聚集体中，促进位于内部的细胞存活。
