Delegan Yanina, Petrikov Kirill, Frantsuzova Ekaterina, Rudenko Natalia, Solomentsev Viktor, Suzina Nataliya, Travkin Vasili, Solyanikova Inna P
Laboratory of Physiology of Microorganisms, Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research of Russian Academy of Sciences" (FRC PSCBR RAS), 142290 Pushchino, Moscow Region, Russia.
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Moscow Region, Russia.
Microorganisms. 2022 Jun 7;10(6):1172. doi: 10.3390/microorganisms10061172.
Microorganisms capable of decomposing hydrophobic substrates in cold climates are of considerable interest both in terms of studying adaptive reactions to low temperatures and in terms of their application in biotechnologies for cleaning up oil spills in a crude-oil polluted soil. The aim of this work was to investigate the genome of S8 and explore behavior traits of this strain grown in the presence of hexadecane. The genome size of strain S8 is 8.78 Mb, of which the chromosome size is 7.75 Mb. The S8 strain contains 2 circular plasmids of 135 kb and 105 kb and a linear plasmid with a size of 788 kb. The analysis of the genome revealed the presence of genes responsible for the degradation of alkanes and synthesis of biosurfactants. The peculiarities of morphology of microbial cells when interacting with a hydrophobic substrate were revealed. An adaptive mechanism responsible in the absence of oxygen for maintaining the process of degradation of hexadecane is discussed. The data obtained show that the strain S8 has great potential to be used in biotechnologies.
在寒冷气候下能够分解疏水性底物的微生物，无论是在研究对低温的适应性反应方面，还是在生物技术中用于清理原油污染土壤中的石油泄漏方面，都具有相当大的研究价值。这项工作的目的是研究S8菌株的基因组，并探索该菌株在十六烷存在下的行为特征。S8菌株的基因组大小为8.78 Mb，其中染色体大小为7.75 Mb。S8菌株包含两个大小分别为135 kb和105 kb的环状质粒以及一个大小为788 kb的线性质粒。基因组分析揭示了负责烷烃降解和生物表面活性剂合成的基因的存在。揭示了微生物细胞与疏水性底物相互作用时的形态学特性。讨论了在无氧条件下维持十六烷降解过程的适应性机制。所获得的数据表明，S8菌株在生物技术中具有巨大的应用潜力。
