Bu Xialian, Zhao Weishan, Li Ming, Li Wenxiang, Wu Shangong, Zou Hong, Wang Guitang
State Key Laboratory of Freshwater Ecology and Biotechnology, and Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Microorganisms. 2022 Aug 15;10(8):1646. doi: 10.3390/microorganisms10081646.
is a facultatively parasitic ciliate, which can opportunistically parasitize on fish gills and fins, and sometimes even cause host mortality. Previous molecular studies of mainly focused on genetic diversity and molecular evolution. There are currently no transcriptome reports studying differences between free-living and parasitic . We addressed this by sequencing transcriptomes of these two lifestyle types using Smart-seq2 and Illumina HiSeq technologies. In total, 1040 differentially expressed genes (DEGs) were identified. Compared with the free-living type, 494 genes of the parasitic type were downregulated and 546 genes were upregulated. These DEGs were identified through BLAST with NCBI-nr, Swiss-Port, and Pfam databases and then annotated by GO enrichment and KEGG pathway analysis. The results showed that parasitism-related genes such as heat shock proteins (HSPs), actin I, and leishmanolysin were significantly upregulated in parasitic . The ciliary-related dynein heavy chain also had a higher expression in parasitic . Furthermore, there were significant differences in the amino acid metabolism, fatty acid metabolism, lipid metabolism, and TCA cycle. This study increases the volume of molecular data available for and contributes to our understanding of the mechanisms underlying the transition from a free-living to a parasitic lifestyle.
是一种兼性寄生的纤毛虫，它可以机会性地寄生于鱼的鳃和鳍上，有时甚至导致宿主死亡。以前对的分子研究主要集中在遗传多样性和分子进化方面。目前尚无研究自由生活型和寄生型之间差异的转录组报告。我们通过使用Smart-seq2和Illumina HiSeq技术对这两种生活方式类型的转录组进行测序来解决这个问题。总共鉴定出1040个差异表达基因（DEG）。与自由生活型相比，寄生型的494个基因下调，546个基因上调。这些DEG通过与NCBI-nr、Swiss-Port和Pfam数据库进行BLAST鉴定，然后通过GO富集和KEGG通路分析进行注释。结果表明，热休克蛋白（HSP）、肌动蛋白I和利什曼溶素等与寄生相关的基因在寄生型中显著上调。与纤毛相关的动力蛋白重链在寄生型中也有较高的表达。此外，在氨基酸代谢、脂肪酸代谢、脂质代谢和三羧酸循环方面存在显著差异。本研究增加了可用于的分子数据量，并有助于我们理解从自由生活向寄生生活方式转变的潜在机制。
