Aydogdu Nida, Ozcelikay Goksu, Ozkan Sibel A
Department of Analytical Chemistry, Faculty of Pharmacy, Afyonkarahisar Health Sciences University, Afyonkarahisar 03218, Turkey.
Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara 06560, Turkey.
Micromachines (Basel). 2022 Aug 19;13(8):1348. doi: 10.3390/mi13081348.
In this research, a glassy carbon electrode (GCE) modified by MWCNT/chitosan NCs/FeO was prepared for the determination of the cephalosporin antibiotic cefditoren (CFT) using adsorptive stripping differential pulse and cyclic voltammetry techniques. The effects of pH, the scan rate, the deposition potential, the accumulation time, and modification agents on the determination of CFT were analyzed. The results showed that the modified electrode significantly increased the oxidation peak current of CFT. Under optimized conditions, the MWCNT/chitosan NCs/FeO/GCE nanosensor exhibited a linear response between 0.2 µM and 10 µM toward CFT. The limit of detection and quantification were determined to be 1.65 nM and 5.50 nM, respectively. Model drugs (cefdinir, cefpodoxime, cephalexin, and ceftazidime compounds) were used to enlighten the CFT oxidation mechanism. Moreover, the nanosensor was used to analyze CFT in a pharmaceutical dosage form and commercial deproteinated human serum samples. The accuracy of these methods was proven in the recovery studies, with values of 96.98 and 98.62% for the pharmaceutical dosage form and commercial deproteinated human serum sample, respectively.
在本研究中，制备了一种由多壁碳纳米管/壳聚糖纳米复合材料/FeO修饰的玻碳电极，采用吸附溶出微分脉冲伏安法和循环伏安法测定头孢菌素类抗生素头孢妥仑（CFT）。分析了pH值、扫描速率、沉积电位、富集时间和修饰剂对CFT测定的影响。结果表明，修饰电极显著提高了CFT的氧化峰电流。在优化条件下，多壁碳纳米管/壳聚糖纳米复合材料/FeO/玻碳电极纳米传感器对CFT在0.2 μM至10 μM之间呈现线性响应。检测限和定量限分别确定为1.65 nM和5.50 nM。使用模型药物（头孢地尼、头孢泊肟、头孢氨苄和头孢他啶化合物）来阐明CFT的氧化机制。此外，该纳米传感器用于分析药物剂型和商业脱蛋白人血清样品中的CFT。这些方法的准确性在回收率研究中得到了验证，药物剂型和商业脱蛋白人血清样品的回收率分别为96.98%和98.62%。
