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弧菌噬菌体阿尔忒弥斯,一种新型感染性噬菌体

Vibrio Phage Artemius, a Novel Phage Infecting .

作者信息

Droubogiannis Stavros, Pavlidi Lydia, Tsertou Maria Ioanna, Kokkari Constantina, Skliros Dimitrios, Flemetakis Emmanouil, Katharios Pantelis

机构信息

Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology & Aquaculture, 71500 Heraklion, Greece.

Department of Biology, School of Sciences and Engineering, University of Crete, 71500 Heraklion, Greece.

出版信息

Pathogens. 2022 Jul 28;11(8):848. doi: 10.3390/pathogens11080848.

Abstract

is an important pathogen of marine animals and has been the target of phage therapy applications in marine aquaculture for many years. Here, we report the isolation and partial characterization of a novel species of the family, the Vibrio phage Artemius. The novel phage was species-specific and could only infect strains of . It could efficiently reduce the growth of the host bacterium at various multiplicities of infection as assessed by an in vitro lysis assay. It had a genome length of 43,349 base pairs. The complete genome has double-stranded DNA with a G + C content of 43.61%. In total, 57 ORFs were identified, of which 19 were assigned a predicted function. A genomic analysis indicated that Vibrio phage Artemius is lytic and does not harbor genes encoding toxins and antibiotic resistance determinants.

摘要

是海洋动物的重要病原体,多年来一直是海水养殖中噬菌体疗法应用的目标。在此,我们报告了弧菌噬菌体阿尔忒弥斯(Vibrio phage Artemius)这一新型噬菌体的分离及部分特性。该新型噬菌体具有种特异性,仅能感染 菌株。通过体外裂解试验评估,它在不同感染复数下均能有效抑制宿主细菌的生长。其基因组长度为43349个碱基对。完整基因组为双链DNA,G + C含量为43.61%。共鉴定出57个开放阅读框(ORF),其中19个被赋予了预测功能。基因组分析表明,弧菌噬菌体阿尔忒弥斯具有裂解性,且不携带编码毒素和抗生素抗性决定簇的基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de18/9416449/63a55cb5a3bb/pathogens-11-00848-g001.jpg

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