Droubogiannis Stavros, Pavlidi Lydia, Tsertou Maria Ioanna, Kokkari Constantina, Skliros Dimitrios, Flemetakis Emmanouil, Katharios Pantelis
Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology & Aquaculture, 71500 Heraklion, Greece.
Department of Biology, School of Sciences and Engineering, University of Crete, 71500 Heraklion, Greece.
Pathogens. 2022 Jul 28;11(8):848. doi: 10.3390/pathogens11080848.
is an important pathogen of marine animals and has been the target of phage therapy applications in marine aquaculture for many years. Here, we report the isolation and partial characterization of a novel species of the family, the Vibrio phage Artemius. The novel phage was species-specific and could only infect strains of . It could efficiently reduce the growth of the host bacterium at various multiplicities of infection as assessed by an in vitro lysis assay. It had a genome length of 43,349 base pairs. The complete genome has double-stranded DNA with a G + C content of 43.61%. In total, 57 ORFs were identified, of which 19 were assigned a predicted function. A genomic analysis indicated that Vibrio phage Artemius is lytic and does not harbor genes encoding toxins and antibiotic resistance determinants.
是海洋动物的重要病原体,多年来一直是海水养殖中噬菌体疗法应用的目标。在此,我们报告了弧菌噬菌体阿尔忒弥斯(Vibrio phage Artemius)这一新型噬菌体的分离及部分特性。该新型噬菌体具有种特异性,仅能感染 菌株。通过体外裂解试验评估,它在不同感染复数下均能有效抑制宿主细菌的生长。其基因组长度为43349个碱基对。完整基因组为双链DNA,G + C含量为43.61%。共鉴定出57个开放阅读框(ORF),其中19个被赋予了预测功能。基因组分析表明,弧菌噬菌体阿尔忒弥斯具有裂解性,且不携带编码毒素和抗生素抗性决定簇的基因。