Lu Yishan, An Guozhen, Wang Xue, Tang Yunlin, Jin Jiangyan, Bao Jialing, Zhou Zeyang
Sate Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715, China.
Pathogens. 2022 Aug 15;11(8):914. doi: 10.3390/pathogens11080914.
Microsporidia are a group of obligated intracellular fungus pathogens. Monocytes and the derivative macrophages are among the most important players in host immunity. The invasion of microsporidia may significantly affect the monocytes maturation and extravasation processes. We utilized a previously established microsporidia infection murine model to investigate the influences of microsporidia () infection on monocyte maturation, releasing into the circulation and extravasation to the inflammation site. Flow cytometry and qPCR analysis were used to compare the monocytes and derivative macrophages isolated from bone marrow, peripheral blood and tissues of -infected and control mice. The results showed that the pro-inflammatory group of CD11bLy-6C monocytes are promoted in -infected mice. Interestingly, the percentage of Ly-6C monocytes from -infected mice are significantly lower in peripheral blood while significantly higher in the inflamed small intestine, together with up-regulated ratio of F4/80 macrophage in small intestine as well. Our findings demonstrated that infection leads to promoted monocytes maturation in bone marrow, up-regulation of extravasation from peripheral blood to inflammation site and maturation into macrophages. Our study is the first systematic analysis of monocytes maturation and trafficking during microsporidia infection, and will provide better understanding of the pathogen-host interactions.
微孢子虫是一类专性细胞内真菌病原体。单核细胞及其衍生的巨噬细胞是宿主免疫中最重要的参与者。微孢子虫的入侵可能会显著影响单核细胞的成熟和外渗过程。我们利用先前建立的微孢子虫感染小鼠模型，研究微孢子虫（）感染对单核细胞成熟、释放到循环系统以及外渗到炎症部位的影响。采用流式细胞术和qPCR分析，比较从感染和对照小鼠的骨髓、外周血和组织中分离出的单核细胞及其衍生的巨噬细胞。结果显示，感染小鼠中促炎性的CD11bLy-6C单核细胞增多。有趣的是，感染小鼠外周血中Ly-6C单核细胞的百分比显著降低，而在发炎的小肠中显著升高，同时小肠中F4/80巨噬细胞的比例也上调。我们的研究结果表明，感染导致骨髓中单核细胞成熟促进，外周血到炎症部位的外渗上调并成熟为巨噬细胞。我们的研究是对微孢子虫感染期间单核细胞成熟和转运的首次系统分析，将有助于更好地理解病原体与宿主的相互作用。
