Akkaoui Sanae, Johansson Anders, Yagoubi Maâmar, Haubek Dorte, El Hamidi Adnane, Rida Sana, Claesson Rolf, Ennibi OumKeltoum
Research laboratory in oral biology and biotechnology, Faculty of dental medicine, Mohammed V University in Rabat, Rabat 10 000, Morocco.
Division of Molecular Periodontology, Department of Odontology, Umeå University, 901 87 Umeå, Sweden.
Pathogens. 2020 Mar 5;9(3):192. doi: 10.3390/pathogens9030192.
In this study, the essential oil of was evaluated for putative antibacterial activity against six clinical strains and five reference strains of , in comparison with some antimicrobials. The chemical composition of the essential oil was analyzed, using chromatography (CG) and gas chromatography-mass spectrometry coupled (CG-MS). The major compounds in the oil were Carvacrol (32.36%), α-terpineol (16.70%), -cymene (16.24%), and Thymol (12.05%). The antimicrobial activity was determined by an agar well diffusion test. A broth microdilution method was used to study the minimal inhibitory concentration (MIC). The minimal bactericidal concentration (MBC) was also determined. The cytotoxicity of the essential oil (IC50) was <125 µg/mL for THP-1 cells, which was high in comparison with different MIC values for the strains. essential oil did not interfere with the neutralizing capacity of against the leukotoxin. In addition, it was shown that the EO had an antibacterial effect against on a similar level as some tested antimicrobials. In view of these findings, we suggest that EO may be used as an adjuvant for prevention and treatment of periodontal diseases associated to . In addition, it can be used together with the previously tested leukotoxin neutralizing .
在本研究中，对[具体名称未给出]的精油针对[具体细菌名称未给出]的六种临床菌株和五种参考菌株的假定抗菌活性进行了评估，并与一些抗菌剂进行了比较。使用色谱法（CG）和气相色谱 - 质谱联用（CG - MS）分析了该精油的化学成分。该精油中的主要化合物为香芹酚（32.36%）、α - 萜品醇（16.70%）、对伞花烃（16.24%）和百里香酚（12.05%）。通过琼脂孔扩散试验测定抗菌活性。采用肉汤微量稀释法研究最低抑菌浓度（MIC）。还测定了最低杀菌浓度（MBC）。该精油对THP - 1细胞的细胞毒性（IC50）<125 µg/mL，与[具体细菌名称未给出]菌株的不同MIC值相比偏高。[具体名称未给出]精油不干扰[具体物质未给出]对[具体毒素未给出]白细胞毒素的中和能力。此外，结果表明[具体名称未给出]精油对[具体细菌名称未给出]的抗菌效果与一些测试的抗菌剂相似。鉴于这些发现，我们建议[具体名称未给出]精油可作为预防和治疗与[具体细菌名称未给出]相关的牙周疾病的佐剂。此外，它可以与先前测试的白细胞毒素中和剂一起使用。
