Yang Xi, Wang Jingyi, Mao Xinguo, Li Chaonan, Li Long, Xue Yinghong, He Liheng, Jing Ruilian
College of Agronomy, Shanxi Agricultural University, Jinzhong 030801, China.
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Plants (Basel). 2022 Aug 9;11(16):2076. doi: 10.3390/plants11162076.
Drought stress frequently occurs, which seriously restricts the production of wheat ( L.). Leaf rolling is a typical physiological phenomenon of plants during drought stress. To understand the genetic mechanism of wheat leaf rolling, we constructed an F segregating population by crossing the slight-rolling wheat cultivar "Aikang 58" (AK58) with the serious-rolling wheat cultivar ″Zhongmai 36″ (ZM36). A combination of bulked segregant analysis (BSA) with Wheat 660K SNP Array was used to identify molecular markers linked to leaf rolling degree. A major locus for leaf rolling degree under drought stress was detected on chromosome 7A. We named this locus (), which was ultimately mapped to a region between 717.82 and 720.18 Mb. Twenty-one genes were predicted in this region, among which the basic helix-loop-helix (bHLH) transcription factor was considered to be the most likely candidate gene for . The TraesCS7A01G543300 is highly homologous to the ICE1 family proteins ICE/SCREAM, SCREAM2 and bHLH093, which control stomatal initiation and development. Two nucleotide variation sites were detected in the promoter region of between the two wheat cultivars. Gene expression assays indicated that was higher expressed in AK58 seedlings than that of ZM36. This research discovered a candidate gene related to wheat leaf rolling under drought stress, which may be helpful for understanding the leaf rolling mechanism and molecular breeding in wheat.
干旱胁迫频繁发生，严重制约了小麦（L.）的产量。叶片卷曲是植物在干旱胁迫期间的一种典型生理现象。为了了解小麦叶片卷曲的遗传机制，我们通过将轻度卷曲小麦品种“矮抗58”（AK58）与重度卷曲小麦品种“中麦36”（ZM36）杂交构建了一个F分离群体。采用混合分组分析法（BSA）与小麦660K SNP芯片相结合的方法来鉴定与叶片卷曲程度相关的分子标记。在7A染色体上检测到一个干旱胁迫下叶片卷曲程度的主效基因座。我们将这个基因座命名为（），最终将其定位到717.82至720.18 Mb之间的一个区域。该区域预测有21个基因，其中基本螺旋-环-螺旋（bHLH）转录因子被认为是最有可能的候选基因。TraesCS7A01G543300与控制气孔起始和发育的ICE1家族蛋白ICE/SCREAM、SCREAM2和bHLH093高度同源。在两个小麦品种之间的启动子区域检测到两个核苷酸变异位点。基因表达分析表明，在AK58幼苗中的表达高于ZM36。本研究发现了一个与干旱胁迫下小麦叶片卷曲相关的候选基因，这可能有助于理解小麦的叶片卷曲机制和分子育种。
