Silva Izabela Caputo Assis, Azevedo Lucas Santos, Castro Ana Hortência Fonsêca, Farias Katyuce de Souza, Zanuncio Vanessa Samúdio Santos, Silva Denise Brentan, Lima Luciana Alves Rodrigues Dos Santos
Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del-Rei (UFSJ), Rua Sebastião Gonçalves Coelho, 400, Chanadour, 35501-296, Divinópolis, MG, Brazil.
Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul (UFMS), Cidade Universitária, Av. Costa e Silva, Pioneiros, 79070-900, Campo Grande, MS, Brazil.
Food Res Int. 2023 Jun;168:112781. doi: 10.1016/j.foodres.2023.112781. Epub 2023 Mar 30.
Smilax brasiliensis Sprengel is a monocotyledon of the Smilacaceae family, native to the Brazilian Cerrado, popularly known as "salsaparrilha" or "japecanga". In this study, the ethanol extract (EE) and the hexane (HEXF), dichloromethane (DCMF), ethyl acetate (ACF), and hydroethanol (HEF) fractions of the stems were obtained. The chemical composition was determined, the contents of phenolic compounds and flavonoids were quantified, and the antioxidant potential and the cytotoxic effect on Artemia salina were evaluated. Fatty acid esters, hydrocarbons, and phytosterols were identified in the HEXF analyzed by gas chromatography - mass spectrometry (GC-MS). The EE and DCMF, ACF, and HEF were analyzed by liquid chromatography coupled to a diode array detector and mass spectrometer (LC-DAD-MS), and the identified constituents included glycosylated (rutin, 3-O-β-galactopyranosyl quercetin, 3-O-β-glucopyranosyl quercetin, O-deoxyhexosyl-hexosyl quercetin, O-deoxyhexosyl-hexosyl kaempferol, O-deoxyhexosyl-hexosyl O-methyl quercetin, and others), and non-glycosylated (quercetin) flavonoids, phenylpropanoids (3-O-E-caffeoyl quinic acid, 5-O-E-caffeoyl quinic acid, O-caffeoyl shikimic acid, and others), neolignan, steroidal saponin (dioscin), and N-feruloyltyramine. The EE, DCMF, and ACF showed high total contents of phenolic compounds (112.99, 175.71, and 524.02 µg of GAE/mg, respectively), and in the ACF and DCMF a great content of flavonoids was also quantified (50.08 and 31.49 µg of QE/mg, respectively). The EE, DCMF, ACF, and HEF exhibited great antioxidant potential by DPPH (IC 1.71 - 32.83 µg/mL) and FRAP (IC 0.63 - 6,71 µg/mL) assays. A maximum cytotoxic activity on A. salina of 60% was observed for the DCMF (LC = 856.17 µg/mL). This study contributes to the phytochemical study of S. brasiliensis since these compounds were identified for the first time in the stems of this species. The S. brasiliensis stems demonstrated to be a rich source of polyphenols compounds and exhibited high antioxidant potential without toxicity. Thus, extract and fractions obtained from the S. brasiliensis stems can be used in food supplements or as natural antioxidants in the food industry.
巴西菝葜是菝葜科的一种单子叶植物，原产于巴西塞拉多，俗称“salsaparrilha”或“japecanga”。在本研究中，获得了该植物茎的乙醇提取物（EE）以及己烷（HEXF）、二氯甲烷（DCMF）、乙酸乙酯（ACF）和氢乙醇（HEF）馏分。测定了其化学成分，对酚类化合物和黄酮类化合物的含量进行了定量，并评估了其抗氧化潜力以及对卤虫的细胞毒性作用。通过气相色谱 - 质谱联用（GC-MS）分析，在HEXF中鉴定出了脂肪酸酯、烃类和植物甾醇。EE、DCMF、ACF和HEF通过液相色谱 - 二极管阵列检测器和质谱联用仪（LC-DAD-MS）进行分析，鉴定出的成分包括糖基化（芦丁、3-O-β-吡喃半乳糖基槲皮素、3-O-β-吡喃葡萄糖基槲皮素、O-脱氧己糖基-己糖基槲皮素、O-脱氧己糖基-己糖基山奈酚、O-脱氧己糖基-己糖基O-甲基槲皮素等）和非糖基化（槲皮素）黄酮类化合物、苯丙素类（3-O-E-咖啡酰奎尼酸、5-O-E-咖啡酰奎尼酸、O-咖啡酰莽草酸等）、新木脂素、甾体皂苷（薯蓣皂苷）和N-阿魏酰酪胺。EE、DCMF和ACF显示出较高的酚类化合物总含量（分别为112.99、175.71和524.02 μg GAE/mg），并且在ACF和DCMF中也定量出了大量的黄酮类化合物（分别为50.08和31.49 μg QE/mg）。通过DPPH（IC 1.71 - 32. The study contributes to the phytochemical study of S. brasiliensis since these compounds were identified for the first time in the stems of this species. The S. brasiliensis stems demonstrated to be a rich source of polyphenols compounds and exhibited high antioxidant potential without toxicity. Thus, extract and fractions obtained from the S. brasiliensis stems can be used in food supplements or as natural antioxidants in the food industry.
83 μg/mL）和FRAP（IC 0.63 - 6.71 μg/mL）测定，EE、DCMF、ACF和HEF表现出很强的抗氧化潜力。观察到DCMF对卤虫的最大细胞毒性活性为60%（LC = 856.17 μg/mL）。这项研究有助于对巴西菝葜进行植物化学研究，因为这些化合物首次在该物种的茎中被鉴定出来。巴西菝葜的茎被证明是多酚化合物的丰富来源，并且具有高抗氧化潜力且无毒性。因此，从巴西菝葜茎中获得的提取物和馏分可用于食品补充剂或作为食品工业中的天然抗氧化剂。
