美国 2009-2016 年轮状病毒株流行趋势：国家轮状病毒株监测系统（NRSSS）结果。

Rotavirus Strain Trends in United States, 2009-2016: Results from the National Rotavirus Strain Surveillance System (NRSSS).

机构信息

Viral Gastroenteritis Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mail Stop G-04, Atlanta, GA 30329, USA.

Indiana University Health Pathology Laboratory, Indiana University, 350 West 11th Street, Indianapolis, IN 46202, USA.

出版信息

Viruses. 2022 Aug 15;14(8):1775. doi: 10.3390/v14081775.

DOI:10.3390/v14081775

PMID:36016397

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9414880/

Abstract

Before the introduction of vaccines, group A rotaviruses (RVA) were the leading cause of acute gastroenteritis in children worldwide. The National Rotavirus Strain Surveillance System (NRSSS) was established in 1996 by the Centers for Disease Control and Prevention (CDC) to perform passive RVA surveillance in the USA. We report the distribution of RVA genotypes collected through NRSSS during the 2009-2016 RVA seasons and retrospectively examine the genotypes detected through the NRSSS since 1996. During the 2009-2016 RVA seasons, 2134 RVA-positive fecal specimens were sent to the CDC for analysis of the VP7 and VP4 genes by RT-PCR genotyping assays and sequencing. During 2009-2011, RVA genotype G3P[8] dominated, while G12P[8] was the dominant genotype during 2012-2016. Vaccine strains were detected in 1.7% of specimens and uncommon/unusual strains, including equine-like G3P[8] strains, were found in 1.9%. Phylogenetic analyses showed limited VP7 and VP4 sequence variation within the common genotypes with 1-3 alleles/lineages identified per genotype. A review of 20 years of NRSSS surveillance showed two changes in genotype dominance, from G1P[8] to G3P[8] and then G3P[8] to G12P[8]. A better understanding of the long-term effects of vaccine use on epidemiological and evolutionary dynamics of circulating RVA strains requires continued surveillance.

摘要

在疫苗问世之前，A 组轮状病毒（RVA）是导致全球儿童急性肠胃炎的主要原因。疾病控制与预防中心（CDC）于 1996 年建立了国家轮状病毒菌株监测系统（NRSSS），以在美国开展被动 RVA 监测。我们报告了通过 NRSSS 在 2009-2016 年 RVA 季节收集的 RVA 基因型分布，并回顾性检查了自 1996 年以来通过 NRSSS 检测到的基因型。在 2009-2016 年 RVA 季节期间，2134 份 RVA 阳性粪便标本被送往 CDC，通过 RT-PCR 基因分型检测和测序分析 VP7 和 VP4 基因。2009-2011 年，RVA 基因型 G3P[8]占主导地位，而 2012-2016 年 G12P[8]则是主要基因型。在 1.7%的标本中检测到疫苗株，而在 1.9%的标本中发现了罕见/不常见的株，包括马源样 G3P[8]株。系统进化分析显示，常见基因型的 VP7 和 VP4 序列变异有限，每个基因型确定了 1-3 个等位基因/谱系。对 20 年 NRSSS 监测的回顾表明，基因型优势发生了两次变化，从 G1P[8]到 G3P[8]，然后从 G3P[8]到 G12P[8]。要更好地了解疫苗使用对循环 RVA 株的流行病学和进化动态的长期影响，需要继续进行监测。