Hu Haichao, Cai Linna, Zhang Tianye, Liu Tingting, Jiang Yaoyao, Liu Hanhong, Lu Qisen, Yang Jian, Chen Jianping
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Viruses. 2022 Aug 16;14(8):1789. doi: 10.3390/v14081789.
Ubiquitination is a major post-translational modification (PTM) involved in almost all eukaryotic biological processes and plays an essential role in plant response to pathogen infection. However, to date, large-scale profiling of the changes in the ubiquitome in response to pathogens, especially viruses, in wheat has not been reported. This study aimed to identify the ubiquitinated proteins involved in (CWMV) infection in wheat using a combination of affinity enrichment and high-resolution liquid chromatography-tandem mass spectroscopy. The potential biological functions of these ubiquitinated proteins were further analyzed using bioinformatics. A total of 2297 lysine ubiquitination sites in 1255 proteins were identified in wheat infected with CWMV, of which 350 lysine ubiquitination sites in 192 proteins were differentially expressed. These ubiquitinated proteins were related to metabolic processes, responses to stress and hormones, plant-pathogen interactions, and ribosome pathways, as assessed via Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. Furthermore, we found that the ubiquitination of Ta14-3-3 and TaHSP90, which are essential components of the innate immune system, was significantly enhanced during CWMV infection, which suggested that ubiquitination modification plays a vital role in the regulatory network of the host response to CWMV infection. In summary, our study puts forward a novel strategy for further probing the molecular mechanisms of CWMV infection. Our findings will inform future research to find better, innovative, and effective solutions to deal with CWMV infection in wheat, which is the most crucial and widely used cereal grain crop.
泛素化是一种主要的翻译后修饰（PTM），几乎参与所有真核生物的生物学过程，并且在植物对病原体感染的反应中起重要作用。然而，迄今为止，尚未见关于小麦中响应病原体尤其是病毒时泛素组变化的大规模分析报道。本研究旨在结合亲和富集和高分辨率液相色谱 - 串联质谱法，鉴定小麦中参与黄瓜花叶病毒（CWMV）感染的泛素化蛋白。利用生物信息学进一步分析了这些泛素化蛋白的潜在生物学功能。在感染CWMV的小麦中，共鉴定出1255个蛋白中的2297个赖氨酸泛素化位点，其中192个蛋白中的350个赖氨酸泛素化位点存在差异表达。通过基因本体论和京都基因与基因组百科全书富集分析评估，这些泛素化蛋白与代谢过程、对胁迫和激素的反应、植物 - 病原体相互作用以及核糖体途径有关。此外，我们发现天然免疫系统的重要组成部分Ta14 - 3 - 3和TaHSP90在CWMV感染期间泛素化显著增强，这表明泛素化修饰在宿主对CWMV感染的反应调控网络中起重要作用。总之，我们的研究提出了一种进一步探究CWMV感染分子机制的新策略。我们的研究结果将为未来研究提供参考，以找到更好的、创新的和有效的解决方案来应对小麦中的CWMV感染，小麦是最重要且广泛种植的谷类作物。
