骨髓增生异常综合征中 microRNA-597 的上调通过抑制 FOSL2 诱导细胞凋亡。
Upregulation of microRNA-597 in myelodysplastic syndromes induces apoptosis through FOSL2 inhibition.
机构信息
Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Republic of Korea.
Department of Premedical Course, Chosun University College of Medicine, Gwangju, Republic of Korea.
出版信息
Eur J Haematol. 2022 Dec;109(6):680-685. doi: 10.1111/ejh.13852. Epub 2022 Sep 7.
INTRODUCTION
Dysregulation of microRNAs (miRNAs) has been associated with the pathophysiology of myelodysplastic syndrome (MDS). Trisomy 8 is the most frequent chromosomal abnormalities in Korean patients with MDS. We investigated the dysregulation of miR-597-5p, located on chromosome 8, which is reported to induce cell death in numerous cancers.
MATERIALS AND METHODS
We compared the expression profiles of miR-597-5p among 65 MDS patients and 11 controls, and analyzed the in vitro effects of miR-597 on leukemic cells using an acute myeloid leukemia cell line transfected with miR-597.
RESULTS
We found that miR-597-5p levels were upregulated 4.05-fold in MDS patients compared to those in controls. In vitro study results demonstrated that transfection with a miR-597 mimic induced apoptosis through downregulation of FOS like 2 (FOSL2).
CONCLUSION
These findings suggest that upregulation of miR-597 induces apoptosis and that miR-597 has a possible role in the pathophysiology of MDS.
简介
微小 RNA（miRNAs）的失调与骨髓增生异常综合征（MDS）的病理生理学有关。8 号染色体三体是韩国 MDS 患者最常见的染色体异常。我们研究了位于 8 号染色体上的 miR-597-5p 的失调，据报道，miR-597-5p 在许多癌症中诱导细胞死亡。
材料与方法
我们比较了 65 例 MDS 患者和 11 例对照者的 miR-597-5p 表达谱，并使用转染 miR-597 的急性髓系白血病细胞系分析 miR-597 对白血病细胞的体外作用。
结果
与对照组相比，MDS 患者的 miR-597-5p 水平上调了 4.05 倍。体外研究结果表明，miR-597 模拟物的转染通过下调 FOS 样 2（FOSL2）诱导细胞凋亡。
结论
这些发现表明 miR-597 的上调诱导细胞凋亡，miR-597 可能在 MDS 的病理生理学中发挥作用。
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