Hino T, Noguchi T, Naito H
Poult Sci. 1987 Mar;66(3):548-51. doi: 10.3382/ps.0660548.
Mucosal cells of the chicken proventriculus were isolated by a collagenase perfusion method and O2 uptake by the isolated cells was measured as an index of the activity of gastric acid secretion. Oxygen consumption was enhanced by histamine; this effect was augmented by the coexistence of isobutylmethylxanthine, an inhibitor of cyclic adenosine 5.-monophosphate (AMP) phosphodiesterase, and suppressed by imidazole, an activator of the enzyme. The action of histamine was inhibited by cimetidine, an antagonist of the histamine H2 receptor. These results indicate that the isolated cells retained the capacity to take up O2, responding to histamine via the H2 receptor and probably the cyclic AMP level. Gizzerosine (2-amino-9-(4-imidazolyl)-7-azanonanoic acid) also stimulated O2 consumption by the isolated cells. The effect of gizzerosine was cancelled by cimetidine, suggesting that the mechanism by which gizzerosine acts on the mucosal cells is similar to that of histamine action. These observations are consistent with our previous presumption that gizzerosine causes gizzard erosion by enhancing gastric acid secretion in chickens.
采用胶原酶灌注法分离鸡腺胃的黏膜细胞，并测定分离细胞的耗氧量，以此作为胃酸分泌活性的指标。组胺可增强耗氧量；环磷酸腺苷（AMP）磷酸二酯酶抑制剂异丁基甲基黄嘌呤的共存可增强此效应，而该酶的激活剂咪唑则可抑制此效应。组胺H2受体拮抗剂西咪替丁可抑制组胺的作用。这些结果表明，分离的细胞保留了摄取氧气的能力，可通过H2受体以及可能通过环磷酸腺苷水平对组胺作出反应。吉泽罗辛（2-氨基-9-（4-咪唑基）-7-氮杂壬酸）也可刺激分离细胞的耗氧量。西咪替丁可消除吉泽罗辛的作用，提示吉泽罗辛作用于黏膜细胞的机制与组胺作用机制相似。这些观察结果与我们之前的推测一致，即吉泽罗辛通过增强鸡的胃酸分泌导致肌胃糜烂。
