In vivo interaction of spironolactone and phenobarbital with cholesterol; effect on cholesterol organ concentration.

In this work, the effect of pretreatment with spironolactone or phenobarbital on female Wistar rats after a chronic administration of cholesterol, upon the cholesterol concentration in blood, brain and liver was examined. It was found that the pretreatment reduced cholesterol in all tissues in the order, brain - plasma - liver. The findings were attributed to an increased biodegradation and disposition of cholesterol. It could also be concluded that after the pretreatment we may have a predominance of the catabolic and/or the disposition rather than of the synthetic and/or absorption processes for the steroid.