Intermale social aggression in rats: suppression by medial hypothalamic lesions independently of enhanced defensiveness or decreased testicular testosterone.

Medial hypothalamic lesions or sham lesions were made in castrated adult male rats with subcutaneous implants of testosterone-filled silastic capsules. Seven days following surgery all animals were given a test of defensiveness (reactivity) toward an experimenter. The following day, groups composed of one lesioned male rat, one sham-lesioned male rat, and one intact female rat were placed in large cages. Beginning two weeks later, unfamiliar intruders were introduced into each colony on a weekly basis and the aggressive behavior of the residents recorded. All 12 of the sham-lesioned animals but only 2 of 12 lesioned animals displayed substantial intermale social aggression toward intruders. Analysis of individual elements of intermale social aggression indicated that the lesioned animals were deficient in attack, bite, and piloerection but not in on-top behavior. The deficit in intermale social aggression was not correlated with defensiveness toward the experimenter or body weight of the lesioned animals. It is argued that the medial hypothalamus plays a role in the modulation of intermale social aggression which is independent of its role in modulating defensiveness or testosterone production. These results also demonstrate that intermale social aggression develops even when testosterone levels are held relatively constant by replacing testicular testosterone with an artificial testosterone source.