编码延伸因子Tu的两个基因均发生改变的大肠杆菌突变体。

Mutants of Escherichia coli altered in both genes coding for the elongation factor Tu.

作者信息

Van de Klundert J A, Van der Meide P H, Van de Putte P, Bosch L

出版信息

Proc Natl Acad Sci U S A. 1978 Sep;75(9):4470-3. doi: 10.1073/pnas.75.9.4470.

DOI:10.1073/pnas.75.9.4470

PMID:360222

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336137/

Abstract

Genetic analysis of a mutant of Escherichia coli resistant to the antibiotic mocimycin is presented. This resistance is due to alterations in both tuf genes coding for the elongation factor Tu. Mocimycin resistance is recessive. Bacteria carryong only one tuf gene from the resistant mutant are still mocimycin sensitive. If the mutant gene is the tufA gene, the seisitive cells can be made resistant through inactivation of the tufB gene by insertion of the bacteriophage milliunits genome. Conditional mocimycin-resistant mutants ban also be isolated when the tufB gene is altered by an amber or a temperature-sensitive mutation. When only the tufB allele from the original mocimycin-resistant mutant is present, inactivation of the wild-type tufA gene fails to give viable mocimycin-resistant progeny. We conclude that the tufA mutant allele codes for a functional mocimycin-resistant EF-Tu, whereas the mutant tufB gene does not code for a functional product.

摘要

本文介绍了对一种抗抗生素莫西霉素的大肠杆菌突变体的遗传分析。这种抗性是由于编码延伸因子Tu的两个tuf基因发生了改变。莫西霉素抗性是隐性的。仅携带来自抗性突变体的一个tuf基因的细菌对莫西霉素仍然敏感。如果突变基因是tufA基因，通过插入噬菌体微小单位基因组使tufB基因失活，可使敏感细胞产生抗性。当tufB基因因琥珀突变或温度敏感突变而改变时，也可分离出条件性莫西霉素抗性突变体。当仅存在来自原始莫西霉素抗性突变体的tufB等位基因时，野生型tufA基因的失活无法产生存活的莫西霉素抗性后代。我们得出结论，tufA突变等位基因编码一种功能性的抗莫西霉素EF-Tu，而突变的tufB基因不编码功能性产物。

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