Radiosensitive antioxidant membrane-bound factors in rat liver microsomes: II. The role of cytosol.

In vitro lipid peroxidation initiated by NADPH/ADP/Fe3+ reveals an alteration of rat liver microsomal membrane at day D+4 after whole-body gamma irradiation (8Gy). This alteration is reversed by Trolox-C 100 microM plus GSH 2mM in association with the soluble supernatant from irradiated or control rats. The antioxidant capacity of the soluble supernatant is not inactivated by gamma irradiation. We conclude that the control of in vivo lipid peroxidation is membranous and cytosolic, and that the preservation of this activity is GSH and Vitamin E dependent.