Thiriot C, Durand P, Jasseron M P, Kergonou J F, Ducousso R
Biochem Int. 1987 Jan;14(1):1-8.
In vitro lipid peroxidation initiated by NADPH/ADP/Fe3+ reveals an alteration of rat liver microsomal antioxidant factors at day D+4 after whole-body gamma irradiation (8Gy). This alteration is partly reversed by GSH, and more efficiently by Trolox C, a water-soluble analog of vitamin E. This reversion by Trolox C, together with the observed 50% decrease in vitamin E content in microsomes of irradiated rats as compared to those of control animals, indicate that Trolox C acts as a free-radical scavenger like and in place of vitamin E. The antioxidant action of Trolox C is not improved in the presence of GSH, which suggests that the former acts earlier than the latter on the autoxidative free-radical chain reactions. Neither GSH, nor Trolox C, nor both antioxidants totally inhibit in vitro lipid peroxidation, which appeals attention on the possible role of extra-microsomal antioxidant factors, especially cytosolic ones.
由NADPH/ADP/Fe3+引发的体外脂质过氧化反应显示，在全身γ射线照射（8Gy）后的第4天（D+4），大鼠肝脏微粒体抗氧化因子发生了改变。谷胱甘肽（GSH）可部分逆转这种改变，而维生素E的水溶性类似物Trolox C则能更有效地逆转。Trolox C的这种逆转作用，以及与对照动物相比，受照射大鼠微粒体中维生素E含量降低50%的观察结果，表明Trolox C像维生素E一样并能替代维生素E发挥自由基清除剂的作用。在存在GSH的情况下，Trolox C的抗氧化作用并未增强，这表明前者在自氧化自由基链式反应中比后者作用更早。GSH、Trolox C或这两种抗氧化剂都不能完全抑制体外脂质过氧化反应，这引起了人们对微粒体外抗氧化因子，尤其是胞质抗氧化因子可能作用的关注。
