Nakamoto T, Quinby G E
Res Commun Chem Pathol Pharmacol. 1987 May;56(2):273-6.
The effects of maternal diabetes on the mandibles in the fetuses of dams given a normal (20% protein) diet vs those given a high protein (40% protein) diet were studied. On day 9, the controls, groups 1 and 3, were injected with citrate buffer and fed a 20% and 40% protein diet, respectively. Groups 2 and 4 were injected with 40 mg/kg B.W. of streptozotocin and pair-fed with groups 1 and 3 with a 20% and 40% protein diet, respectively. On day 22, fetuses were removed. Dissected mandibles were weighed and analyzed for DNA, protein, Ca and hydroxyproline contents. Some dams in each group were injected with either 45Ca or 14C proline to study 45Ca uptake and collagen synthesis. The body and mandibular weights of the fetuses from diabetic (D) dams were smaller than those of the non-diabetic (ND) group in both diets. The protein and hydroxyproline contents of the mandible in the D 20% group was less than the ND. Hydroxyproline content from the D 40% group was less than the ND. The Ca content in the D 40% group was less than the ND. The present study indicates that metabolism in the bone of fetuses whose D dams were fed a high protein diet is affected beneficially in some parameters but not in others.
研究了母体糖尿病对给予正常（20%蛋白质）饮食的母鼠所产胎儿下颌骨的影响，并与给予高蛋白（40%蛋白质）饮食的母鼠所产胎儿下颌骨进行了比较。在第9天，对照组（第1组和第3组）分别注射柠檬酸盐缓冲液，并给予20%和40%蛋白质的饮食。第2组和第4组分别注射40mg/kg体重的链脲佐菌素，并分别与第1组和第3组配对喂养，给予20%和40%蛋白质的饮食。在第22天，取出胎儿。解剖后的下颌骨称重，并分析其DNA、蛋白质、钙和羟脯氨酸含量。每组中的一些母鼠注射了45Ca或14C脯氨酸，以研究45Ca摄取和胶原蛋白合成。在两种饮食中，糖尿病（D）母鼠所产胎儿的体重和下颌骨重量均小于非糖尿病（ND）组。D 20%组下颌骨的蛋白质和羟脯氨酸含量低于ND组。D 40%组的羟脯氨酸含量低于ND组。D 40%组的钙含量低于ND组。本研究表明，D母鼠喂食高蛋白饮食时，其胎儿骨骼中的某些参数代谢受到有益影响，但其他参数则未受影响。
