Berry L R, Shaye R, Nakamoto T
Proc Soc Exp Biol Med. 1987 Jun;185(2):141-6. doi: 10.3181/00379727-185-42528.
Dams with 7 pups each were randomly assigned to two different diets. Twelve dams were fed a normal (20%) protein diet and were divided into two groups of 4 and 8 animals. Pups from group 1 (n = 28) were injected with citrate buffer as a control. Pups from group 2 (n = 56) were injected with streptozotocin. Twelve additional dams were fed a 40% protein diet. They were also divided into two groups of 4 and 8 animals. Pups from group 3 (n = 28) were injected with citrate buffer as a control. Pups from group 4 (n = 56) were injected with streptozotocin. Forty-eight hours later, diabetic status was determined using Dextrostix. On Day 15, pups were injected with [14C]proline to determine collagen synthesis and 45Ca to study mineralization. After the pups were killed, blood glucose levels were determined. Then mandibles were removed. Milk from each dam was also collected after injection of oxytocin. At the time of killing, blood glucose levels in diabetic pups were less than earlier levels, though still higher than those of controls on either diet. The weights of body and mandible, collagen contents, and the total calcium contents in the diabetic group were in general less than those of the nondiabetic group on the 20 and 40% protein diets. 45Ca uptake in the diabetic group was significantly increased compared with those of the nondiabetic rats on both diets. The percentage reduction in the mandibles of diabetic rats from those of nondiabetic rats on the 40% protein diets was consistently less than that of animals on the 20% protein diets. The higher protein contents of the maternal milk in the 40% protein group may partly be responsible for the smaller impairment of mandibular development in the diabetic over nondiabetic animals. It is concluded that maternal low-carbohydrate high-protein diets will play indirectly a beneficial role in the development of the mandibles of diabetic newborns.
每组有7只幼崽的母鼠被随机分配到两种不同的饮食组。12只母鼠喂食正常（20%）蛋白质饮食，并分为4只和8只动物的两组。第1组（n = 28）的幼崽注射柠檬酸盐缓冲液作为对照。第2组（n = 56）的幼崽注射链脲佐菌素。另外12只母鼠喂食40%蛋白质饮食。它们也分为4只和8只动物的两组。第3组（n = 28）的幼崽注射柠檬酸盐缓冲液作为对照。第4组（n = 56）的幼崽注射链脲佐菌素。48小时后，使用葡萄糖试纸测定糖尿病状态。在第15天，给幼崽注射[14C]脯氨酸以测定胶原蛋白合成，并注射45Ca以研究矿化作用。幼崽被处死后，测定血糖水平。然后取出下颌骨。在注射催产素后，还收集了每只母鼠的乳汁。处死时，糖尿病幼崽的血糖水平低于早期水平，但仍高于两种饮食组的对照组。在20%和40%蛋白质饮食组中，糖尿病组的体重、下颌骨重量、胶原蛋白含量和总钙含量总体上低于非糖尿病组。与两种饮食组的非糖尿病大鼠相比，糖尿病组的45Ca摄取量显著增加。在40%蛋白质饮食组中，糖尿病大鼠下颌骨相对于非糖尿病大鼠的减少百分比始终低于20%蛋白质饮食组的动物。40%蛋白质组母鼠乳汁中较高的蛋白质含量可能部分是糖尿病动物下颌骨发育受损小于非糖尿病动物的原因。得出的结论是，母体低碳水化合物高蛋白饮食将间接对糖尿病新生儿下颌骨的发育起到有益作用。
