Future Energy and Innovation Laboratory, Central European Institute of Technology, Brno University of Technology, Purkynova 123, Brno, 61200, Czech Republic.
Faculty of Electrical Engineering and Computer Science, VSB - Technical University of Ostrava, 17. Listopadu 2172/15, Ostrava, 70800, Czech Republic.
Small. 2022 Sep;18(39):e2202600. doi: 10.1002/smll.202202600. Epub 2022 Aug 26.
Antibiotics are antimicrobial substances that can be used for preventive and therapeutic purposes in humans and animals. Their overdose usage has led to uncontrolled release to the environment, contributing significantly to the development of antimicrobial resistance phenomena. Here, enzyme-immobilized self-propelled zinc oxide (ZnO) microrobots are proposed to effectively target and degrade the released antibiotics in water bodies. Specifically, the morphology of the microrobots is tailored via the incorporation of Au during the synthetic process to lead the light-controlled motion into having on/off switching abilities. The microrobots are further modified with laccase enzyme by physical adsorption, and the immobilization process is confirmed by enzymatic activity measurements. Oxytetracycline (OTC) is used as a model of veterinary antibiotics to investigate the enzyme-immobilized microrobots for their removal capacities. The results demonstrate that the presence of laccase on the microrobot surfaces can enhance the removal of antibiotics via oxidation. This concept for immobilizing enzymes on self-propelled light-driven microrobots leads to the effective removal of the released antibiotics from water bodies with an environmentally friendly strategy.
抗生素是可用于人类和动物预防和治疗目的的抗菌物质。它们的过量使用导致了对环境的失控释放，这对抗菌药物耐药性现象的发展有重大贡献。在这里，提出了酶固定自推进氧化锌（ZnO）微米机器人，以有效地针对和降解水体中释放的抗生素。具体而言，通过在合成过程中掺入 Au 来调整微米机器人的形态，使其光控运动具有开/关切换能力。然后通过物理吸附将漆酶进一步修饰到微米机器人上，并通过酶活性测量来确认固定化过程。使用土霉素（OTC）作为兽用抗生素的模型来研究固定酶的微米机器人对其去除能力。结果表明，在微米机器人表面上存在漆酶可以通过氧化增强抗生素的去除。将酶固定在自推进光驱动微米机器人上的这种概念导致了从水体中以环保的策略有效去除释放的抗生素。
