Biochemistry Department, College of Medicine, Taif University, Taif, Saudi Arabia.
Neurology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Genet Test Mol Biomarkers. 2022 Jul-Aug;26(7-8):398-407. doi: 10.1089/gtmb.2021.0301.
Diabetes mellitus is a known risk factor for stroke and may be linked to poorer post-stroke outcomes. However, the underlying molecular mechanisms remain to be fully identified. In this study we assessed the association of the lncRNA Nuclear enriched abundant transcript 1 ()'s expression and its target miRNA-124 with acute ischemic stroke (AIS) in type II diabetic patients (T2DM). Diabetic patients with stroke, non-diabetics with stroke, diabetics without stroke, and controls were recruited. and miR-124 expression levels in plasma samples from the participants were investigated using real-time reverse transcription-polymerase chain reaction (RT-qPCR). C reactive protein (CRP) and tumor necrosis factor alpha (TNF-α) were measured using an enzyme linked immunosorbent assay (ELISA) technique. In the DM+AIS group, expression was considerably higher, compared with AIS group and with control group. In comparison to the AIS-only patients, DM patients and controls, miR-124 expression was considerably lower in the DM+AIS group. was shown to have good predictive value for AIS risk in diabetics, based on Receiver Operating Characteristic (ROC) curve analysis. In both the DM+AIS and the AIS group, NEAT1 levels was strongly linked with the National Institutes of Health Stroke Scale (NIHSS) score. Also, a significant positive correlation was observed between NEAT1 expression and the inflammatory markers CRP and TNF-α and significant negative association with miRNA-124 in patient groups. In diabetic patients, the lncRNA may influence the incidence, severity, inflammation, and prognosis of AIS. expression levels could be used as a diagnostic marker of stroke in diabetic patients.
糖尿病是中风的已知危险因素，可能与中风后预后较差有关。然而，其潜在的分子机制仍有待充分确定。在这项研究中，我们评估了长链非编码 RNA 核丰富丰富转录物 1（）的表达及其靶 miRNA-124 与 2 型糖尿病患者（T2DM）急性缺血性中风（AIS）的相关性。招募了患有中风的糖尿病患者、患有中风的非糖尿病患者、没有中风的糖尿病患者和对照组。使用实时逆转录聚合酶链反应（RT-qPCR）检测参与者血浆样本中的和 miR-124 表达水平。使用酶联免疫吸附测定（ELISA）技术测量 C 反应蛋白（CRP）和肿瘤坏死因子α（TNF-α）。在 DM+AIS 组中，与 AIS 组和对照组相比，表达水平显著升高。与仅 AIS 患者相比，DM 患者和对照组的 DM+AIS 组中 miR-124 表达水平显著降低。基于接受者操作特征（ROC）曲线分析，显示对于糖尿病患者的 AIS 风险，具有良好的预测价值。在 DM+AIS 和 AIS 组中，NEAT1 水平与美国国立卫生研究院中风量表（NIHSS）评分密切相关。此外，在患者组中，还观察到 NEAT1 表达与炎症标志物 CRP 和 TNF-α之间存在显著正相关，与 miRNA-124 之间存在显著负相关。在糖尿病患者中，lncRNA 可能影响 AIS 的发生、严重程度、炎症和预后。表达水平可作为糖尿病患者中风的诊断标志物。
