Department of Pharmacy, Zhongnan Hospital of Wuhan University, Dong-Hu Road #169, Wuhan, Hubei, 430071, P.R. China.
Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
J Mol Neurosci. 2024 Apr 3;74(2):36. doi: 10.1007/s12031-024-02207-5.
After ischemic stroke, microRNAs (miRNAs) participate in various processes, including immune responses, inflammation, and angiogenesis. Diabetes is a key factor increasing the risk of ischemic stroke; however, the regulatory pattern of miRNAs at different stages of diabetic stroke remains unclear. This study comprehensively analyzed the miRNA expression profiles in diabetic mice at 1, 3, and 7 days post-reperfusion following the middle cerebral artery occlusion (MCAO). We identified differentially expressed (DE) miRNAs in diabetic stroke and found significant dysregulation of some novel miRNAs (novel_mir310, novel_mir89, and novel_mir396) post-stroke. These DEmiRNAs were involved in apoptosis and the formation of tight junctions. Finally, we identified three groups of time-dependent DE miRNAs (miR-6240, miR-135b-3p, and miR-672-5p). These have the potential to serve as biomarkers of diabetic stroke. These findings provide a new perspective for future research, emphasizing the dynamic changes in miRNA expression after diabetic stroke and offering potential candidates as biomarkers for future clinical applications.
缺血性脑卒中后，microRNAs（miRNAs）参与多种过程，包括免疫反应、炎症和血管生成。糖尿病是增加缺血性脑卒中风险的关键因素；然而，糖尿病性脑卒中不同阶段miRNAs 的调控模式尚不清楚。本研究全面分析了大脑中动脉闭塞（MCAO）后再灌注 1、3 和 7 天的糖尿病小鼠中的 miRNA 表达谱。我们鉴定了糖尿病性脑卒中的差异表达（DE）miRNAs，并发现了一些新型 miRNA（novel_mir310、novel_mir89 和 novel_mir396）在脑卒中后的显著失调。这些 DEmiRNAs 参与细胞凋亡和紧密连接的形成。最后，我们鉴定了三组时间依赖性 DEmiRNAs（miR-6240、miR-135b-3p 和 miR-672-5p）。这些有可能作为糖尿病性脑卒中的生物标志物。这些发现为未来的研究提供了一个新的视角，强调了糖尿病性脑卒中后 miRNA 表达的动态变化，并为未来的临床应用提供了潜在的候选生物标志物。
