Department of Life Sciences Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Department of Life Sciences Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Biomater Adv. 2022 Sep;140:213084. doi: 10.1016/j.bioadv.2022.213084. Epub 2022 Aug 20.
Fomes fomentarius is a medicinal fungus used in traditional Chinese medicine to treat various illnesses. Antidiabetic effects of F. fomentarius extracts have been reported recently. In this study, F. fomentarius extracellular polysaccharide (PS) was prepared, and then to enhance its antidiabetic effects, NaSeO was added to the culture medium, and selenium-polysaccharide (PS-Se) was obtained. Also, solid lipid nanoparticles containing PS (SLN-PS) and PS-Se (SLN-PS-Se) were synthesized by the microemulsion method to compare their effects with free polysaccharides in streptozotocin (STZ) diabetic rats. Optimized SLNs had a size of 170.5 nm and drug loading of 9.27 %. EDS analysis confirmed that Se presence in PS-Se. Characterization analyses such as FTIR, DSC, TGA, and XRD suggested that SLNs have good thermal stability and crystalline nature. Release of PS from SLNs demonstrated sustained profile, and MTT assay proved that PSs and SLNs have no cytotoxicity. Furthermore, oral administration of PS, PS-Se, SLN-PS, and SLN-PS-Se for 28 days to diabetic rats significantly declined blood glucose by 48.24 %, 49.96 %, 55.50 %, and 60.47 %, respectively. Also, insulin secretion and body weight improved, and HbA1c levels decreased. Treatment by PS, PS-Se, SLN-PS, and SLN-PS-Se alleviated lipid profiles, liver enzymes, and serum proteins. Liver anti-oxidant parameters and histopathological observation of the liver, pancreas, and kidney confirmed that F. fomentarius PSs and SLNs have antidiabetic impacts. Moreover, supplementation of PS with selenium improves its anti-hyperglycemic effects. Finally, SLN-PS and SLN-PS-Se showed a higher antidiabetic impact than free PS and PS-Se.
裂蹄木层孔菌是一种药用真菌，用于治疗各种疾病。最近报道了裂蹄木层孔菌提取物的降血糖作用。在这项研究中，制备了裂蹄木层孔菌胞外多糖（PS），然后为了增强其降血糖作用，在培养基中加入亚硒酸钠，得到硒-多糖（PS-Se）。此外，采用微乳液法合成了载 PS（SLN-PS）和 PS-Se（SLN-PS-Se）的固体脂质纳米粒，以比较它们在链脲佐菌素（STZ）糖尿病大鼠中的作用与游离多糖的作用。优化的 SLN 粒径为 170.5nm，载药量为 9.27%。EDS 分析证实 PS-Se 中存在硒。傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）、热重分析（TGA）和 X 射线衍射（XRD）等表征分析表明，SLN 具有良好的热稳定性和结晶性。PS 从 SLN 中的释放呈持续释放，MTT 试验证明 PS 和 SLN 无细胞毒性。此外，PS、PS-Se、SLN-PS 和 SLN-PS-Se 经口给药 28 天，可使糖尿病大鼠的血糖分别降低 48.24%、49.96%、55.50%和 60.47%。此外，胰岛素分泌和体重增加，HbA1c 水平降低。PS、PS-Se、SLN-PS 和 SLN-PS-Se 治疗可改善血脂谱、肝酶和血清蛋白。肝脏抗氧化参数和肝、胰、肾的组织病理学观察证实，裂蹄木层孔菌 PS 和 SLN 具有降血糖作用。此外，硒与 PS 联合补充可提高其降血糖作用。最后，SLN-PS 和 SLN-PS-Se 比游离 PS 和 PS-Se 具有更高的降血糖作用。
