Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wheat Disease Biology Research Station for Central China, Wuhan, China.
Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wheat Disease Biology Research Station for Central China, Wuhan, China.
Int J Biol Macromol. 2022 Nov 1;220:985-997. doi: 10.1016/j.ijbiomac.2022.08.124. Epub 2022 Aug 23.
Calmodulin (CaM) and calmodulin-like (CML) proteins are the most prominent calcium (Ca) sensing proteins involved in Ca-signaling processes. However, the function of these calcium sensors in wheat remains unclear. In this study, 15 TaCAMs and 113 TaCMLs were identified from the wheat reference genome. The analysis of cis-acting elements and expression patterns showed that TaCAMs might play an important role in response to abiotic and biotic stresses. TaCAM2-D gene was found to be significantly upregulated under drought and salt stresses, and thus, it was selected to further explore the biological function. Moreover, TaCAM2-D was observed to be localized in the nucleus, membrane and cytoplasm. Overexpression of TaCAM2-D in Arabidopsis conferred greater tolerance to drought and salt. The prediction analysis, the yeast two-hybrid analysis, and bimolecular fluorescence complementation assay indicated that TaCAM2-D interacted with TaMPK8, which is one of the wheat mitogen-activated protein kinases. Thus, the current study provides insights into the understanding of the TaCAM and TaCML genes in wheat.
钙调蛋白（CaM）和钙调蛋白样蛋白（CML）是参与钙信号转导过程的最主要的钙感应蛋白。然而，这些钙传感器在小麦中的功能尚不清楚。本研究从小麦参考基因组中鉴定出了 15 个 TaCAMs 和 113 个 TaCMLs。顺式作用元件和表达模式分析表明，TaCAMs 可能在非生物和生物胁迫响应中发挥重要作用。发现 TaCAM2-D 基因在干旱和盐胁迫下显著上调，因此选择其进一步探索生物学功能。此外，观察到 TaCAM2-D 定位于细胞核、膜和细胞质。在拟南芥中过表达 TaCAM2-D 赋予其对干旱和盐胁迫更强的耐受性。预测分析、酵母双杂交分析和双分子荧光互补测定表明 TaCAM2-D 与 TaMPK8 相互作用，TaMPK8 是一种小麦丝裂原活化蛋白激酶。因此，本研究为理解小麦中的 TaCAM 和 TaCML 基因提供了新视角。
