Institute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan, 430064, China.
National Key Laboratory of Rice Biology/Institute of Crop Sciences, Zhejiang University, Hangzhou, 310058, China.
Plant Physiol Biochem. 2023 Sep;202:107921. doi: 10.1016/j.plaphy.2023.107921. Epub 2023 Aug 1.
Ferritin not only regulates the plant's iron content but also plays a significant role in the plant's development and resistance to oxidative damage. However, the role of the FER family in wheat has not been systematically elucidated. In this study, 39 FERs identified from wheat and its ancestral species were clustered into two subgroups, and gene members from the same group contain relatively conservative protein models. The structural analyses indicated that the gene members from the same group contained relatively conserved protein models. The cis-acting elements and expression patterns analysis suggested that TaFERs might play an important role combating to abiotic and biotic stresses. In the transcriptional analysis, the TaFER5D-1 gene was found to be significantly up-regulated under drought and salt stresses and was, therefore, selected to further explore the biological functions Moreover, the GFP expression assay revealed the subcellular localization of TaFER5D-1 proteins in the chloroplast, nucleus, membrane and cytoplasm. Over-expression of TaFER5D-1 in transgenic Arabidopsis lines conferred greater tolerance to drought and salt stress. According to the qRT-PCR data, TaFER5D-1 gene over-expression increased the expression of genes related to root development (Atsweet-17 and AtRSL4), iron storage (AtVIT1 and AtYSL1), and stress response (AtGolS1 and AtCOR47). So it is speculated that TaFER5D-1 could improve stress tolerance by promoting root growth, iron storage, and stress-response ability. Thus, the current study provides insight into the role of TaFER genes in wheat.
铁蛋白不仅调节植物的铁含量，而且在植物的发育和对氧化损伤的抗性中起着重要作用。然而，FER 家族在小麦中的作用尚未得到系统阐明。本研究从小麦及其祖先物种中鉴定出 39 个 FER，将其分为两个亚组，同一组的基因成员含有相对保守的蛋白模型。结构分析表明，同一组的基因成员含有相对保守的蛋白模型。顺式作用元件和表达模式分析表明，TaFER 可能在应对非生物和生物胁迫方面发挥重要作用。在转录分析中，发现 TaFER5D-1 基因在干旱和盐胁迫下显著上调，因此选择进一步探索其生物学功能。此外，GFP 表达分析显示 TaFER5D-1 蛋白在叶绿体、核、膜和细胞质中的亚细胞定位。TaFER5D-1 在转基因拟南芥系中的过表达赋予了对干旱和盐胁迫更大的耐受性。根据 qRT-PCR 数据，TaFER5D-1 基因过表达增加了与根发育（Atsweet-17 和 AtRSL4）、铁储存（AtVIT1 和 AtYSL1）和应激反应（AtGolS1 和 AtCOR47）相关的基因的表达。因此，推测 TaFER5D-1 通过促进根生长、铁储存和应激反应能力来提高胁迫耐受性。因此，本研究为 TaFER 基因在小麦中的作用提供了新的认识。
